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Regulatory T Cells

Overview of attention for book
Cover of 'Regulatory T Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Regulatory T Cells: History and Perspective
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    Chapter 2 In vitro Treg suppression assays.
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    Chapter 3 Generation of T Cell Hybridomas from Naturally Occurring FoxP3+ Regulatory T Cells
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    Chapter 4 In Vitro and In Vivo Analyses of Regulatory T Cell Suppression of CD8+ T Cells
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    Chapter 5 Flow cytometric profiling of mature and developing regulatory T cells in the thymus.
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    Chapter 6 ChIP-on-Chip for FoxP3
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    Chapter 7 Live Imaging of Dendritic Cell–Treg Cell Interactions
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    Chapter 8 Genetic Tools for Analysis of FoxP3+ Regulatory T Cells In Vivo
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    Chapter 9 In vivo Treg suppression assays.
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    Chapter 10 In Vivo Depletion of FoxP3+ Tregs Using the DEREG Mouse Model
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    Chapter 11 Antigen-Specific Induction of Regulatory T Cells In Vivo and In Vitro
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    Chapter 12 In Vitro Expansion of Alloantigen-Specific Regulatory T Cells and Their Use in Prevention of Allograft Rejection
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    Chapter 13 Analysis of Human FOXP3+ Treg Cells Phenotype and Function
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    Chapter 14 Depletion of human regulatory T cells.
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    Chapter 15 Assessment of Suppressive Capacity by Human Regulatory T Cells Using a Reproducible, Bi-Directional CFSE-Based In Vitro Assay
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    Chapter 16 Measurement of Proliferation and Disappearance of Regulatory T Cells in Human Studies Using Deuterium-Labeled Glucose
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    Chapter 17 Flow cytometric detection of human regulatory T cells.
Attention for Chapter 17: Flow cytometric detection of human regulatory T cells.
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Chapter title
Flow cytometric detection of human regulatory T cells.
Chapter number 17
Book title
Regulatory T Cells
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61737-979-6_17
Pubmed ID
21287341
Book ISBNs
978-1-61737-978-9, 978-1-61737-979-6
Authors

Fazekas de St Groth B, Zhu E, Asad S, Lee L, Barbara Fazekas de St Groth, Erhua Zhu, Suzanne Asad, Loretta Lee

Editors

George Kassiotis, Adrian Liston

Abstract

Tregs are absolutely required for the maintenance of self tolerance in mouse and man. Major abnormalities in Treg number or function cause rare but fatal syndromes with autoimmune, allergic and inflammatory features. Whether subtle Treg abnormalities contribute to the pathogenesis of sporadic autoimmune, allergic and immunoinflammatory diseases in man remains controversial. Robust methods for identifying and isolating human Tregs in patients and healthy controls are essential if we are to understand their role in these increasingly common diseases. We have outlined below a flow cytometric technique to detect and isolate the entire human Treg population based on expression of CD4, CD25, and CD127. Use of a number of additional antibodies for defining subsets within the Treg compartment is described. For analysis, anti-Foxp3 can be added to the cocktail, but the necessity for fixation and permeabilisation may reduce the signal from other antibodies.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 54 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 54 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 22%
Student > Bachelor 11 20%
Student > Ph. D. Student 10 19%
Student > Master 6 11%
Student > Doctoral Student 1 2%
Other 4 7%
Unknown 10 19%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 22%
Immunology and Microbiology 10 19%
Medicine and Dentistry 8 15%
Biochemistry, Genetics and Molecular Biology 6 11%
Engineering 3 6%
Other 3 6%
Unknown 12 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 April 2013.
All research outputs
#20,187,333
of 22,703,044 outputs
Outputs from Methods in molecular biology
#9,848
of 13,076 outputs
Outputs of similar age
#169,947
of 180,443 outputs
Outputs of similar age from Methods in molecular biology
#201
of 232 outputs
Altmetric has tracked 22,703,044 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,076 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 232 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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