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Muscle Stem Cells

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Cover of 'Muscle Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Muscle Stem Cells: A Model System for Adult Stem Cell Biology
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    Chapter 2 Isolation of Muscle Stem Cells from Mouse Skeletal Muscle
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    Chapter 3 Primary Mouse Myoblast Purification using Magnetic Cell Separation
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    Chapter 4 Isolation, Culture, and Immunostaining of Skeletal Muscle Myofibers from Wildtype and Nestin-GFP Mice as a Means to Analyze Satellite Cell
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    Chapter 5 Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors
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    Chapter 6 Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells
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    Chapter 7 Muscle Interstitial Cells: A Brief Field Guide to Non-satellite Cell Populations in Skeletal Muscle
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    Chapter 8 Isolation and Characterization of Vessel-Associated Stem/Progenitor Cells from Skeletal Muscle
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    Chapter 9 Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture
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    Chapter 10 Single Cell Gene Expression Profiling of Skeletal Muscle-Derived Cells
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    Chapter 11 Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle
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    Chapter 12 Transplantation of Skeletal Muscle Stem Cells
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    Chapter 13 Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells
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    Chapter 14 Monitoring Autophagy in Muscle Stem Cells
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    Chapter 15 Mimicking Muscle Stem Cell Quiescence in Culture: Methods for Synchronization in Reversible Arrest
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    Chapter 16 Methods for Observing and Quantifying Muscle Satellite Cell Motility and Invasion In Vitro
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    Chapter 17 Effects of Macrophage Conditioned-Medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion
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    Chapter 18 Optimization of Satellite Cell Culture Through Biomaterials
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    Chapter 19 Systematic Identification of Genes Regulating Muscle Stem Cell Self-Renewal and Differentiation
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    Chapter 20 Bioinformatics for Novel Long Intergenic Noncoding RNA (lincRNA) Identification in Skeletal Muscle Cells
Attention for Chapter 17: Effects of Macrophage Conditioned-Medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion
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Chapter title
Effects of Macrophage Conditioned-Medium on Murine and Human Muscle Cells: Analysis of Proliferation, Differentiation, and Fusion
Chapter number 17
Book title
Muscle Stem Cells
Published in
Methods in molecular biology, March 2017
DOI 10.1007/978-1-4939-6771-1_17
Pubmed ID
Book ISBNs
978-1-4939-6769-8, 978-1-4939-6771-1
Authors

Marielle Saclier, Marine Theret, Rémi Mounier, Bénédicte Chazaud

Editors

Eusebio Perdiguero, DDW Cornelison

Abstract

Skeletal muscle is a highly plastic tissue, which is able to regenerate after an injury. Effective and complete regeneration requires interactions between myogenic precursor cells and several cell types such as macrophages . Bone marrow derived macrophages in mouse and monocyte-derived macrophages in human are useful tools to obtain macrophage populations that may be specifically activated/polarized in vitro (e.g., pro-inflammatory, anti-inflammatory, and alternatively activated macrophages ). In vitro , human or murine primary myogenic cells recapitulate the adult myogenesis program through proliferation, myogenic differentiation, and fusion. Macrophages being highly secreting cells, they act on various biological processes including adult myogenesis . Here, we present protocols to analyze in vitro the effect of macrophage-secreted factors on muscle cell proliferation or differentiation in both mouse and human.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 7%
Unknown 13 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 29%
Student > Ph. D. Student 3 21%
Student > Bachelor 2 14%
Student > Master 1 7%
Lecturer > Senior Lecturer 1 7%
Other 0 0%
Unknown 3 21%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 29%
Biochemistry, Genetics and Molecular Biology 3 21%
Medicine and Dentistry 2 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Engineering 1 7%
Other 0 0%
Unknown 3 21%