Chapter title |
Localized Surface Plasmon Resonance (LSPR)-Coupled Fiber-Optic Nanoprobe for the Detection of Protein Biomarkers
|
---|---|
Chapter number | 1 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6848-0_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6846-6, 978-1-4939-6848-0
|
Authors |
Jianjun Wei, Zheng Zeng, Yongbin Lin |
Editors |
Avraham Rasooly, Ben Prickril |
Abstract |
Here is presented a miniaturized, fiber-optic (FO) nanoprobe biosensor based on the localized surface plasmon resonance (LSPR) at the reusable dielectric-metallic hybrid interface with a robust, gold nano-disk array at the fiber end facet. The nanodisk array is directly fabricated using electron beam lithography (EBL) and metal lift-off process. The free prostate-specific antigen (f-PSA) has been detected with a mouse anti-human prostate-specific antigen (PSA) monoclonal antibody (mAb) as a specific receptor linked with a self-assembled monolayer (SAM) at the LSPR-FO facet surfaces. Experimental investigation and data analysis found near field refractive index (RI) sensitivity at ~226 nm/RIU with the LSPR-FO nanoprobe, and demonstrated the lowest limit of detection (LOD) at 100 fg/mL (~3 fM) of f-PSA in PBS solutions. The SAM shows insignificant nonspecific binding to the target biomarkers in the solution. The control experimentation using 5 mg/mL bovine serum albumin in PBS and nonspecific surface test shows the excellent specificity and selectivity in the detection of f-PSA in PBS. These results indicate important progress toward a miniaturized, multifunctional fiber-optic technology that integrates informational communication and sensing function for developing a high-performance, label-free, point-of-care (POC) device. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 26 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Master | 4 | 15% |
Student > Bachelor | 4 | 15% |
Professor | 2 | 8% |
Researcher | 2 | 8% |
Student > Ph. D. Student | 2 | 8% |
Other | 4 | 15% |
Unknown | 8 | 31% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 23% |
Materials Science | 2 | 8% |
Unspecified | 1 | 4% |
Nursing and Health Professions | 1 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 2 | 8% |
Unknown | 13 | 50% |