Chapter title |
Reflectometric Interference Spectroscopy
|
---|---|
Chapter number | 13 |
Book title |
Biosensors and Biodetection
|
Published in |
Methods in molecular biology, March 2017
|
DOI | 10.1007/978-1-4939-6848-0_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6846-6, 978-1-4939-6848-0
|
Authors |
Guenther Proll, Goran Markovic, Peter Fechner, Florian Proell, Guenter Gauglitz |
Editors |
Avraham Rasooly, Ben Prickril |
Abstract |
Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance. The advantages of direct optical detection depend on a sophisticated surface chemistry resulting negligible nonspecific binding and high loading with recognition sites at the biopolymer sensitive layer of the transducer. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy (RIfS). A standard protocol for a binding inhibition assay is given. It overcomes principal problems of any direct optical detection technique. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 14% |
Researcher | 3 | 14% |
Student > Ph. D. Student | 2 | 9% |
Student > Master | 2 | 9% |
Professor | 2 | 9% |
Other | 3 | 14% |
Unknown | 7 | 32% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 18% |
Agricultural and Biological Sciences | 2 | 9% |
Chemistry | 2 | 9% |
Physics and Astronomy | 2 | 9% |
Unspecified | 1 | 5% |
Other | 2 | 9% |
Unknown | 9 | 41% |