Chapter title |
Delivery of siRNAs to Cancer Cells via Bacteria
|
---|---|
Chapter number | 7 |
Book title |
RNA Interference
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-1538-5_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1537-8, 978-1-4939-1538-5
|
Authors |
Omar Ahmed, Andrea Krühn, Hermann Lage, Ahmed, Omar, Krühn, Andrea, Lage, Hermann |
Abstract |
RNA interference (RNAi) technology is a promising approach for efficient silencing of a particular gene for cancer gene therapy. However, the main obstacle for the development of RNAi-based therapeutic approaches is the delivery of the RNAi effector molecules to target cells. One promising strategy to surmount this challenge is the application of nonpathogenic bacteria as a delivery vector to target cells. In this chapter, the design of invasive Escherichia coli is described. The strain carries a plasmid encoding short hairpin RNAs (shRNAs), a protein (invasin) necessary for endocytotic absorption of the bacteria by target cells, and listeriolysin O required for the lysis of endocytotic vesicles within the target cells. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 5 | 28% |
Student > Ph. D. Student | 4 | 22% |
Student > Master | 3 | 17% |
Student > Doctoral Student | 1 | 6% |
Other | 1 | 6% |
Other | 2 | 11% |
Unknown | 2 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 6 | 33% |
Medicine and Dentistry | 3 | 17% |
Biochemistry, Genetics and Molecular Biology | 2 | 11% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Immunology and Microbiology | 1 | 6% |
Other | 3 | 17% |
Unknown | 2 | 11% |