Title |
Response of human dental pulp cells to a silver-containing PLGA/TCP-nanofabric as a potential antibacterial regenerative pulp-capping material
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Published in |
BMC Oral Health, February 2017
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DOI | 10.1186/s12903-017-0348-7 |
Pubmed ID | |
Authors |
Barbara Cvikl, Samuel C. Hess, Richard J. Miron, Hermann Agis, Dieter Bosshardt, Thomas Attin, Patrick R. Schmidlin, Adrian Lussi |
Abstract |
Damage or exposure of the dental pulp requires immediate therapeutic intervention. This study assessed the biocompatibility of a silver-containing PLGA/TCP-nanofabric scaffold (PLGA/Ag-TCP) in two in vitro models, i.e. the material adapted on pre-cultured cells and cells directly cultured on the material, respectively. Collagen saffolds with and without hyaluronan acid (Coll-HA; Coll) using both cell culturing methods and cells growing on culture plates served as reference. Cell viability and proliferation were assessed after 24, 48, and 72 h based on formazan formation and BrdU incorporation. Scaffolds were harvested. Gene expression of interleukin(IL)-6, tumor necrosis factor (TNF)-alpha, and alkaline phosphatase (AP) was assessed 24 h after stimulation. In both models formazan formation and BrdU incorporation was reduced by PLGA/Ag-TCP on dental pulp cells, while no significant reduction was found in cells with Coll and Coll-HA. Cells with PLGA/Ag-TCP for 72 h showed similar relative BrdU incorporation than cells stimulated with Coll and Coll-HA. A prominent increase in the pro-inflammatory genes IL-6 and TNF-α was observed when cells were cultured with PLGA/Ag-TCP compared to the other groups. This increase was parallel with a slight increase in AP expression. Overall, no differences between the two culture methods were observed. PLGA/Ag-TCP decreased viability and proliferation rate of human dental pulp cells and increased the pro-inflammatory capacity and alkaline phosphatase expression. Whether these cellular responses observed in vitro translate into pulp regeneration in vivo will be assessed in further studies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 62 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 8 | 13% |
Student > Master | 8 | 13% |
Student > Bachelor | 5 | 8% |
Other | 4 | 6% |
Student > Doctoral Student | 4 | 6% |
Other | 16 | 26% |
Unknown | 17 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 21 | 34% |
Materials Science | 4 | 6% |
Agricultural and Biological Sciences | 4 | 6% |
Biochemistry, Genetics and Molecular Biology | 3 | 5% |
Chemical Engineering | 2 | 3% |
Other | 8 | 13% |
Unknown | 20 | 32% |