Chapter title |
Genetic Transformation of the Model Green Alga Chlamydomonas reinhardtii
|
---|---|
Chapter number | 4 |
Book title |
Transgenic Plants
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-558-9_4 |
Pubmed ID | |
Book ISBNs |
978-1-61779-557-2, 978-1-61779-558-9
|
Authors |
Ralph Bock, Juliane Neupert, Ning Shao, Yinghong Lu, Neupert, Juliane, Shao, Ning, Lu, Yinghong, Bock, Ralph |
Abstract |
Over the past three decades, the single-celled green alga Chlamydomonas reinhardtii has become an invaluable model organism in plant biology and an attractive production host in biotechnology. The genetic transformation of Chlamydomonas is relatively simple and efficient, but achieving high expression levels of foreign genes has remained challenging. Here, we provide working protocols for algal cultivation and transformation as well as for selection and analysis of transgenic algal clones. We focus on two commonly used transformation methods for Chlamydomonas: glass bead-assisted transformation and particle gun-mediated (biolistic) transformation. In addition, we describe available tools for promoting efficient transgene expression and highlight important considerations for designing transformation vectors. |
X Demographics
Geographical breakdown
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 2 | 2% |
United Kingdom | 1 | <1% |
Brazil | 1 | <1% |
Greece | 1 | <1% |
Mexico | 1 | <1% |
Unknown | 102 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 27 | 25% |
Student > Ph. D. Student | 22 | 20% |
Student > Master | 15 | 14% |
Student > Bachelor | 14 | 13% |
Professor | 4 | 4% |
Other | 14 | 13% |
Unknown | 12 | 11% |
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Agricultural and Biological Sciences | 56 | 52% |
Biochemistry, Genetics and Molecular Biology | 28 | 26% |
Environmental Science | 2 | 2% |
Chemistry | 2 | 2% |
Business, Management and Accounting | 2 | 2% |
Other | 7 | 6% |
Unknown | 11 | 10% |