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Morpholino Oligomers

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Cover of 'Morpholino Oligomers'

Table of Contents

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    Book Overview
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    Chapter 1 Invention and Early History of Morpholinos: From Pipe Dream to Practical Products
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    Chapter 2 Making a Morpholino Experiment Work: Controls, Favoring Specificity, Improving Efficacy, Storage, and Dose
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    Chapter 3 Aggregation and Disaggregation of Morpholino Oligomers in Solution
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    Chapter 4 End-Modifications on Morpholino Oligos
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    Chapter 5 Inducible Inhibition of Gene Function with Photomorpholinos
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    Chapter 6 Blocking Zebrafish MicroRNAs with Morpholinos
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    Chapter 7 Using Morpholinos to Examine Gene Function During Fin Regeneration
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    Chapter 8 Using Morpholinos to Probe Gene Networks in Sea Urchin
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    Chapter 9 Manipulating Gene Expression in the Chick Embryo
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    Chapter 10 Inhibition of Bacterial Growth by Peptide-Conjugated Morpholino Oligomers
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    Chapter 11 Use of Translation Blocking Morpholinos for Gene Knockdown in Giardia lamblia
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    Chapter 12 Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos
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    Chapter 13 Targeting Functional Noncoding RNAs
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    Chapter 14 Use of Morpholino Oligomers for Pretargeting
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    Chapter 15 Diagnostic Applications of Morpholinos and Label-Free Electrochemical Detection of Nucleic Acids
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    Chapter 16 Intranasal Delivery of Peptide-Morpholinos to Knockdown Influenza Host Factors in Mice
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    Chapter 17 Systemic Delivery of Morpholinos to Skip Multiple Exons in a Dog Model of Duchenne Muscular Dystrophy
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    Chapter 18 In Vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina
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    Chapter 19 Intracerebroventricular Delivery in Mice for Motor Neuron Diseases
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    Chapter 20 Delivery of Morpholino Antisense Oligonucleotides to a Developing Ovine Conceptus via Luminal Injection into a Ligated Uterine Horn
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    Chapter 21 Surface Plasmon Resonance-Based Concentration Determination Assay: Label-Free and Antibody-Free Quantification of Morpholinos
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    Chapter 22 Ultrasensitive Hybridization-Based ELISA Method for the Determination of Phosphorodiamidate Morpholino Oligonucleotides in Biological samples
Attention for Chapter 18: In Vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina
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Chapter title
In Vivo and Explant Electroporation of Morpholinos in the Developing Mouse Retina
Chapter number 18
Book title
Morpholino Oligomers
Published in
Methods in molecular biology, April 2017
DOI 10.1007/978-1-4939-6817-6_18
Pubmed ID
Book ISBNs
978-1-4939-6815-2, 978-1-4939-6817-6
Authors

Devi Krishna Priya Karunakaran, Rahul Kanadia

Editors

Hong M. Moulton, Jon D. Moulton

Abstract

Neonatal in vivo electroporations and retinal explant electroporations have been widely employed in understanding the effects of loss or gain of function of protein-coding genes in retinal development. Here, we describe a rapid and efficient delivery of morpholinos to add another tool to perturb gene expression during mouse retinal development.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 50%
Student > Bachelor 1 25%
Student > Doctoral Student 1 25%
Readers by discipline Count As %
Medicine and Dentistry 2 50%
Agricultural and Biological Sciences 1 25%
Pharmacology, Toxicology and Pharmaceutical Science 1 25%