| Chapter title |
Aggregation and Disaggregation of Morpholino Oligomers in Solution
|
|---|---|
| Chapter number | 3 |
| Book title |
Morpholino Oligomers
|
| Published in |
Methods in molecular biology, April 2017
|
| DOI | 10.1007/978-1-4939-6817-6_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6815-2, 978-1-4939-6817-6
|
| Authors |
Garrick Chow, Paul A. Morcos, Hong M. Moulton |
| Editors |
Hong M. Moulton, Jon D. Moulton |
| Abstract |
Morpholino oligomers are effective antisense molecules to regulate gene expression and the US FDA has approved a Morpholino drug for the treatment of Duchenne muscular dystrophy. However, it has been observed that the antisense activities of aqueous solutions of some Morpholinos decrease over time. We hypothesize that the decreased activity is caused by the formation of soluble aggregates of the Morpholinos. Here, we analyzed three Morpholino sequences by size exclusion chromatography and found two of them have over time formed soluble aggregates in water. The degree of aggregation is sequence-, temperature-, and time-dependent. We describe a simple procedure for detecting and breaking down the aggregates to return the Morpholinos to their monomeric forms. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 25% |
| Student > Bachelor | 2 | 17% |
| Student > Master | 2 | 17% |
| Professor | 1 | 8% |
| Lecturer > Senior Lecturer | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 2 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 25% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 17% |
| Chemistry | 2 | 17% |
| Medicine and Dentistry | 1 | 8% |
| Chemical Engineering | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 3 | 25% |