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Lysosomes

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Cover of 'Lysosomes'

Table of Contents

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    Book Overview
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    Chapter 1 SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS. - PubMed - NCBI
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    Chapter 2 Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics
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    Chapter 3 Analysis of N- and O-Glycosylation of Lysosomal Glycoproteins
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    Chapter 4 Analyzing Lysosome-Related Organelles by Electron Microscopy
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    Chapter 5 Microscopic Analysis of Lysosomal Membrane Permeabilization
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    Chapter 6 Quantitative Co-Localization and Pattern Analysis of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets
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    Chapter 7 Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues
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    Chapter 8 Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy-Dependent Recovery
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    Chapter 9 Delivery of Cargo to Lysosomes Using GNeosomes
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    Chapter 10 Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles
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    Chapter 11 Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells
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    Chapter 12 Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry
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    Chapter 13 Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes
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    Chapter 14 Using the MEROPS Database for Investigation of Lysosomal Peptidases, Their Inhibitors, and Substrates
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    Chapter 15 Next-Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of LysoPlex
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    Chapter 16 Gelatin Zymography Using Leupeptin for the Detection of Various Cathepsin L Forms
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    Chapter 17 Methods for Determination of α-Glycosidase, β-Glycosidase, and α-Galactosidase Activities in Dried Blood Spot Samples
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    Chapter 18 Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi
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    Chapter 19 Lysosomal Biology in Cancer
Attention for Chapter 8: Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy-Dependent Recovery
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Chapter title
Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy-Dependent Recovery
Chapter number 8
Book title
Lysosomes
Published in
Methods in molecular biology, April 2017
DOI 10.1007/978-1-4939-6934-0_8
Pubmed ID
Book ISBNs
978-1-4939-6932-6, 978-1-4939-6934-0
Authors

Otomo, Takanobu, Yoshimori, Tamotsu, Takanobu Otomo, Tamotsu Yoshimori

Editors

Karin Öllinger, Hanna Appelqvist

Abstract

Selective autophagy recognizes specific targets, including damaged mitochondria (mitophagy), aggregated proteins (aggrephagy), and invading bacteria (xenophagy) to engulf by isolation membrane, and degrades toxic materials within lysosomes. We recently revealed that a membrane-damaged lysosome itself also becomes a target of autophagy and named this process lysophagy. In this chapter, we describe methods for monitoring lysophagy; detecting lysosomal damage by staining of galectin and study the subsequent autophagic process in cultured mammalian cells.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 20 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 15%
Researcher 3 15%
Student > Master 3 15%
Student > Bachelor 1 5%
Other 1 5%
Other 1 5%
Unknown 8 40%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 30%
Agricultural and Biological Sciences 3 15%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Immunology and Microbiology 1 5%
Neuroscience 1 5%
Other 0 0%
Unknown 8 40%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2017.
All research outputs
#20,418,183
of 22,968,808 outputs
Outputs from Methods in molecular biology
#9,923
of 13,142 outputs
Outputs of similar age
#270,071
of 310,341 outputs
Outputs of similar age from Methods in molecular biology
#231
of 282 outputs
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So far Altmetric has tracked 13,142 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 282 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.