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Chapter title |
ESPRIT: A Method for Defining Soluble Expression Constructs in Poorly Understood Gene Sequences
|
---|---|
Chapter number | 4 |
Book title |
Heterologous Gene Expression in E.coli
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6887-9_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6885-5, 978-1-4939-6887-9
|
Authors |
Philippe J. Mas, Darren J. Hart |
Editors |
Nicola A. Burgess-Brown |
Abstract |
Production of soluble, purifiable domains or multi-domain fragments of proteins is a prerequisite for structural biology and other applications. When target sequences are poorly annotated, or when there are few similar sequences available for alignments, identification of domains can be problematic. A method called expression of soluble proteins by random incremental truncation (ESPRIT) addresses this problem by high-throughput automated screening of tens of thousands of enzymatically truncated gene fragments. Rare soluble constructs are identified by experimental screening, and the boundaries revealed by DNA sequencing. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 18 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 22% |
Student > Postgraduate | 3 | 17% |
Student > Ph. D. Student | 2 | 11% |
Student > Bachelor | 2 | 11% |
Student > Master | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 6 | 33% |
Agricultural and Biological Sciences | 3 | 17% |
Nursing and Health Professions | 1 | 6% |
Medicine and Dentistry | 1 | 6% |
Chemistry | 1 | 6% |
Other | 0 | 0% |
Unknown | 6 | 33% |