Chapter title |
N- and C-Terminal Truncations to Enhance Protein Solubility and Crystallization: Predicting Protein Domain Boundaries with Bioinformatics Tools
|
---|---|
Chapter number | 2 |
Book title |
Heterologous Gene Expression in E.coli
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6887-9_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6885-5, 978-1-4939-6887-9, 978-1-4939-6885-5, 978-1-4939-6887-9
|
Authors |
Christopher D. O. Cooper, Brian D. Marsden |
Editors |
Nicola A. Burgess-Brown |
Abstract |
Soluble protein expression is a key requirement for biochemical and structural biology approaches to study biological systems in vitro. Production of sufficient quantities may not always be achievable if proteins are poorly soluble which is frequently determined by physico-chemical parameters such as intrinsic disorder. It is well known that discrete protein domains often have a greater likelihood of high-level soluble expression and crystallizability. Determination of such protein domain boundaries can be challenging for novel proteins. Here, we outline the application of bioinformatics tools to facilitate the prediction of potential protein domain boundaries, which can then be used in designing expression construct boundaries for parallelized screening in a range of heterologous expression systems. |
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