| Chapter title |
Assay Methods for ACS Activity and ACS Phosphorylation by MAP Kinases In Vitro and In Vivo
|
|---|---|
| Chapter number | 6 |
| Book title |
Ethylene Signaling
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6854-1_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6852-7, 978-1-4939-6854-1
|
| Authors |
Xiaomin Han, Guojing Li, Shuqun Zhang |
| Editors |
Brad M. Binder, G. Eric Schaller |
| Abstract |
Ethylene, a gaseous phytohormone, has profound effects on plant growth, development, and adaptation to the environment. Ethylene-regulated processes begin with the induction of ethylene biosynthesis. There are two key steps in ethylene biosynthesis. The first is the biosynthesis of 1-aminocyclopropane-1-carboxylic acid (ACC) from S-Adenosyl-Methionine (SAM), a common precursor in many metabolic pathways, which is catalyzed by ACC synthase (ACS). The second is the oxidative cleavage of ACC to form ethylene under the action of ACC oxidase (ACO). ACC biosynthesis is the committing and generally the rate-limiting step in ethylene biosynthesis. As a result, characterizing the cellular ACS activity and understanding its regulation are important. In this chapter, we detail the methods used to measure, (1) the enzymatic activity of both recombinant and native ACS proteins, and (2) the phosphorylation of ACS protein by mitogen-activated protein kinases (MAPKs) in vivo and in vitro. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor | 1 | 17% |
| Student > Postgraduate | 1 | 17% |
| Student > Doctoral Student | 1 | 17% |
| Student > Master | 1 | 17% |
| Unknown | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 50% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 17% |
| Unknown | 2 | 33% |