Chapter title |
Quantitative microbial metatranscriptomics.
|
---|---|
Chapter number | 17 |
Book title |
Environmental Microbiology
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-62703-712-9_17 |
Pubmed ID | |
Book ISBNs |
978-1-62703-711-2, 978-1-62703-712-9
|
Authors |
Scott Gifford, Brandon Satinsky, Mary Ann Moran, Gifford, Scott, Satinsky, Brandon, Moran, Mary Ann |
Abstract |
The direct retrieval and sequencing of environmental RNA is emerging as a powerful technique to elucidate the in situ activities of microbial communities. Here we provide a metatranscriptomic protocol describing environmental sample collection, rRNA depletion, mRNA amplification, cDNA synthesis, and bioinformatic analysis. In addition, the preparation of internal RNA standards and their addition to the sample are discussed, providing a method by which transcript numbers can be expressed as absolute abundances in the environment and more readily compared to other biogeochemical and ecological measurements. |
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United States | 4 | 40% |
Germany | 1 | 10% |
Canada | 1 | 10% |
Unknown | 4 | 40% |
Demographic breakdown
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Scientists | 8 | 80% |
Members of the public | 2 | 20% |
Mendeley readers
Geographical breakdown
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United States | 2 | 5% |
Unknown | 41 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 15 | 35% |
Student > Ph. D. Student | 8 | 19% |
Student > Master | 5 | 12% |
Professor > Associate Professor | 3 | 7% |
Other | 2 | 5% |
Other | 5 | 12% |
Unknown | 5 | 12% |
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Biochemistry, Genetics and Molecular Biology | 5 | 12% |
Immunology and Microbiology | 4 | 9% |
Environmental Science | 3 | 7% |
Engineering | 2 | 5% |
Other | 3 | 7% |
Unknown | 7 | 16% |