| Chapter title |
Quantitative PCR for Detection of mRNA and gDNA in Environmental Isolates.
|
|---|---|
| Chapter number | 3 |
| Book title |
Environmental Microbiology
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-62703-712-9_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-711-2, 978-1-62703-712-9
|
| Authors |
Brzoska AJ, Hassan KA, Brzoska, Anthony J, Hassan, Karl A, Anthony J. Brzoska, Karl A. Hassan, Brzoska, Anthony J., Hassan, Karl A. |
| Editors |
Ian T. Paulsen, Andrew J. Holmes |
| Abstract |
Quantitative PCR is used to gauge the abundance of specific nucleic acid species within purified samples. Due to its high sensitivity and minimal operation costs, this method is routinely applied in modern molecular bioscience laboratories. Nonetheless, all quantitative PCR experiments must include several carefully designed, yet simple, controls to ensure the reliability of the analyses. The aim of this chapter is to provide basic quantitative PCR methods, from primer design through data analysis, that are generally applicable to studies in microbiology. These methods allow the abundance of targeted RNA or DNA molecules to be determined in nucleic acid samples purified from a variety of biological sources. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 43% |
| Student > Ph. D. Student | 2 | 29% |
| Student > Master | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 57% |
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Unknown | 1 | 14% |