Chapter title |
Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331
|
---|---|
Chapter number | 15 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7183-1_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7182-4, 978-1-4939-7183-1
|
Authors |
García-Fernández, Julia, Martínez, Igor, Fernández-Cabezón, Lorena, Felpeto-Santero, Carmen, García, José-Luis, Galán, Beatriz, Julia García-Fernández, Igor Martínez, Lorena Fernández-Cabezón, Carmen Felpeto-Santero, José-Luis García, Beatriz Galán |
Abstract |
The C19 steroid 1,4-androstadiene-3,17-dione (androstadienedione, ADD) is an added value product used as a synthon in the pharmaceutical industry for the commercial production of corticosteroids, mineralocorticoids, oral contraceptives, and other pharmaceutical steroids. Phytosterol biotransformation catalyzed by microbial whole cells is actually a very well-established research area in white biotechnology. The protocol below provides detailed information on ADD production by the mutant CECT 8331 of Mycobacterium smegmatis mc(2)155 using phytosterols as raw material in a lab scale. This protocol describes the bioconversion of phytosterols into ADD in a single fermentation step. |
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Unknown | 2 | 29% |