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Human Respiratory Syncytial Virus

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Cover of 'Human Respiratory Syncytial Virus'

Table of Contents

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    Book Overview
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    Chapter 1 Human Respiratory Syncytial Virus: An Introduction
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    Chapter 2 Human Respiratory Syncytial Virus
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    Chapter 3 Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay
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    Chapter 4 Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
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    Chapter 5 Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells
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    Chapter 6 Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique
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    Chapter 7 Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection
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    Chapter 8 Host Factors Modulating RSV Infection: Use of Small Interfering RNAs to Probe Functional Importance
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    Chapter 9 In Vitro Modeling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs)
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    Chapter 10 Reverse Genetics of Respiratory Syncytial Virus
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    Chapter 11 Use of Minigenome Systems to Study RSV Transcription
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    Chapter 12 Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics
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    Chapter 13 A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets
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    Chapter 14 Human Respiratory Syncytial Virus
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    Chapter 15 Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus
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    Chapter 16 Human Respiratory Syncytial Virus
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    Chapter 17 Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus
Attention for Chapter 4: Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
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Chapter title
Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
Chapter number 4
Book title
Human Respiratory Syncytial Virus
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3687-8_4
Pubmed ID
Book ISBNs
978-1-4939-3685-4, 978-1-4939-3687-8
Authors

Samadhan J. Jadhao, Larry J. Anderson

Abstract

Enzyme immunoassays (EIAs) to detect and quantify antibodies against respiratory syncytial virus (RSV) and RSV proteins in human plasma or sera are described. The first EIA uses RSV lysate antigens produced in HEp-2 cell line. The second EIA uses RSV F or G gene-expressed antigen in HEp-2 cells. The third EIA uses 30-amino acid synthetic peptides from central conserved region of G protein of RSV A2 or RSV B1 virus and a peptide from the SARS CoV nucleoprotein as a negative control peptide. All three EIAs have been evaluated for detecting and quantifying the respective antibodies in human sera or plasma.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 7 33%
Librarian 2 10%
Other 2 10%
Researcher 2 10%
Student > Ph. D. Student 2 10%
Other 1 5%
Unknown 5 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 10%
Nursing and Health Professions 2 10%
Medicine and Dentistry 2 10%
Immunology and Microbiology 2 10%
Environmental Science 1 5%
Other 3 14%
Unknown 9 43%