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Human Respiratory Syncytial Virus

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Cover of 'Human Respiratory Syncytial Virus'

Table of Contents

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    Book Overview
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    Chapter 1 Human Respiratory Syncytial Virus: An Introduction
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    Chapter 2 Human Respiratory Syncytial Virus
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    Chapter 3 Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay
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    Chapter 4 Detection of RSV Antibodies in Human Plasma by Enzyme Immunoassays
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    Chapter 5 Secretory Expression and Purification of Respiratory Syncytial Virus G and F Proteins in Human Cells
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    Chapter 6 Development of Human Monoclonal Antibodies Against Respiratory Syncytial Virus Using a High Efficiency Human Hybridoma Technique
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    Chapter 7 Respiratory Syncytial Virus (RSV): Neutralizing Antibody, a Correlate of Immune Protection
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    Chapter 8 Host Factors Modulating RSV Infection: Use of Small Interfering RNAs to Probe Functional Importance
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    Chapter 9 In Vitro Modeling of RSV Infection and Cytopathogenesis in Well-Differentiated Human Primary Airway Epithelial Cells (WD-PAECs)
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    Chapter 10 Reverse Genetics of Respiratory Syncytial Virus
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    Chapter 11 Use of Minigenome Systems to Study RSV Transcription
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    Chapter 12 Screening for Host Factors Directly Interacting with RSV Protein: Microfluidics
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    Chapter 13 A Proteomic-Based Workflow Using Purified Respiratory Syncytial Virus Particles to Identify Cellular Factors as Drug Targets
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    Chapter 14 Human Respiratory Syncytial Virus
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    Chapter 15 Mouse and Cotton Rat Models of Human Respiratory Syncytial Virus
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    Chapter 16 Human Respiratory Syncytial Virus
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    Chapter 17 Evaluation of the Adaptive Immune Response to Respiratory Syncytial Virus
Attention for Chapter 3: Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay
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Chapter title
Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay
Chapter number 3
Book title
Human Respiratory Syncytial Virus
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3687-8_3
Pubmed ID
Book ISBNs
978-1-4939-3685-4, 978-1-4939-3687-8
Authors

Patricia A. Jorquera, Ralph A. Tripp

Abstract

One of the most commonly used approaches for determining the quantity of infectious RSV particles in a given sample is the plaque assay. RSV infectious particles can be quantified by various direct and indirect methods. Here, we explain two simple methods for RSV titration: plaque assay and immunostaining assay.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 5 18%
Researcher 5 18%
Professor > Associate Professor 3 11%
Student > Ph. D. Student 3 11%
Other 2 7%
Other 3 11%
Unknown 7 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 21%
Immunology and Microbiology 5 18%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Chemical Engineering 1 4%
Medicine and Dentistry 1 4%
Other 2 7%
Unknown 11 39%