Chapter title |
Cell Reprogramming
|
---|---|
Chapter number | 6 |
Book title |
Cell Reprogramming
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2848-4_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2847-7, 978-1-4939-2848-4
|
Authors |
Malaver-Ortega, Luis F, Taheri-Ghahfarokhi, Amir, Sumer, Huseyin, Malaver-Ortega, Luis F., Luis F. Malaver-Ortega, Amir Taheri-Ghahfarokhi, Huseyin Sumer |
Abstract |
Nuclear reprogramming technologies in general and induced pluripotent stem cells (iPSCs) in particular have opened the door to a vast number of practical applications in regenerative medicine and biotechnology. It also represents a possible alternative to the still evasive achievement of embryonic stem cells (ESCs) isolation from refractory species such as Bos. taurus. Herein, we described a protocol for bovine iPSCs (biPSCs) generation and characterization. The protocol is based on the overexpression of the exogenous transcription factors NANOG, OCT4, SOX2, KLF4 and c-MYC, using a pantropic retroviral system. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 25% |
Student > Bachelor | 1 | 13% |
Other | 1 | 13% |
Unknown | 4 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 38% |
Agricultural and Biological Sciences | 2 | 25% |
Unknown | 3 | 38% |