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Mendeley readers
| Chapter title |
Generation of Chimeras from Porcine Induced Pluripotent Stem Cells
|
|---|---|
| Chapter number | 14 |
| Book title |
Cell Reprogramming
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-2848-4_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-2847-7, 978-1-4939-2848-4
|
| Authors |
Franklin D. West, Steve L. Terlouw, John R. Dobrinsky, Yangqing Lu, Erin T. Jordan, Steven L. Stice |
| Abstract |
Pig induced pluripotent stem cells (piPSCs) offer a great opportunity and a number of advantages in the generation of transgenic animals. These immortalized cells can undergo multiple rounds of genetic modifications (e.g., gene knock-in, knockout) and selection leading to animals that have optimized traits of biomedical or agricultural interests. In this chapter we describe the production and characterization of piPSCs, microinjection of piPSCs into embryos, embryo transfer and production of chimeric animals based on successful protocols. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 2 | 29% |
| Researcher | 2 | 29% |
| Student > Bachelor | 1 | 14% |
| Student > Master | 1 | 14% |
| Student > Ph. D. Student | 1 | 14% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 43% |
| Agricultural and Biological Sciences | 2 | 29% |
| Immunology and Microbiology | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |