Chapter title |
Sequential Formation of Regioselective Disulfide Bonds in Synthetic Peptides with Multiple Disulfide Bonds
|
---|---|
Chapter number | 5 |
Book title |
Peptide Synthesis and Applications
|
Published in |
Methods in molecular biology, January 2013
|
DOI | 10.1007/978-1-62703-544-6_5 |
Pubmed ID | |
Book ISBNs |
978-1-62703-543-9, 978-1-62703-544-6
|
Authors |
Fazel Shabanpoor, Mohammed Akhter Hossain, Feng Lin, John D. Wade, Shabanpoor, Fazel, Hossain, Mohammed Akhter, Lin, Feng, Wade, John D. |
Abstract |
Numerous methods have been developed for the formation of disulfide bonds in recombinant DNA-derived and chemically synthesized peptides and proteins, but only a few have found widespread acceptance. The choice of method(s) for formation of disulfide in synthetic peptides and proteins needs to be tailored for each individual polypeptide in such a way so that the reaction conditions are selective, efficient, and safe and give the maximum yield. Here we describe the sequential formation of three disulfide bonds regioselectively which has been optimized for the synthesis of two-chained, heterodimeric polypeptide members of the insulin-relaxin superfamily. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Researcher | 1 | 20% |
Lecturer | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 2 | 40% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 20% |
Unknown | 2 | 40% |