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Mendeley readers
Chapter title |
Computational Sequence Design with R2oDNA Designer
|
---|---|
Chapter number | 18 |
Book title |
Mammalian Synthetic Promoters
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7223-4_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7221-0, 978-1-4939-7223-4
|
Authors |
James T. MacDonald, Velia Siciliano |
Abstract |
Recently developed DNA assembly methods have enabled the rapid and simultaneous assembly of multiple parts to create complex synthetic gene circuits. A number of groups have proposed the use of computationally designed orthogonal spacer sequences to guide the ordered assembly of parts using overlap-directed or homologous recombination-based methods. This approach is particularly useful for assembling multiple parts with repetitive elements. Orthogonal spacer sequences (sometimes called UNSs-unique nucleotide sequences) also have a number of other potential uses including in the design of synthetic promoters regulated by novel regulatory elements. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 47% |
Student > Ph. D. Student | 2 | 12% |
Student > Bachelor | 1 | 6% |
Student > Master | 1 | 6% |
Professor > Associate Professor | 1 | 6% |
Other | 0 | 0% |
Unknown | 4 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 29% |
Agricultural and Biological Sciences | 4 | 24% |
Immunology and Microbiology | 2 | 12% |
Nursing and Health Professions | 1 | 6% |
Neuroscience | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 18% |