Chapter title |
A Photoaffinity Labeling-Based Chemoproteomics Strategy for Unbiased Target Deconvolution of Small Molecule Drug Candidates
|
---|---|
Chapter number | 1 |
Book title |
Proteomics for Drug Discovery
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7201-2_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7200-5, 978-1-4939-7201-2
|
Authors |
Jason R. Thomas, Scott M. Brittain, Jennifer Lipps, Luis Llamas, Rishi K. Jain, Markus Schirle |
Abstract |
The combination of photoaffinity labeling (PAL) and quantitative chemoproteomics enables the comprehensive, unbiased determination of protein interaction profiles to support target identification of bioactive small molecules. This approach is amenable to cells in culture and compatible with pharmacologically relevant transmembrane target classes like G-protein coupled receptors and ions channels which have been notoriously hard to access by conventional chemoproteomics approaches. Here, we describe a strategy that combines PAL probe titration and competition with excess parental compounds with the goal of enabling the identification of specific interactors as well as assessing the functional relevance of a binding event for the phenotype under investigation. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 35 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 23% |
Researcher | 7 | 20% |
Student > Bachelor | 4 | 11% |
Student > Master | 4 | 11% |
Professor | 1 | 3% |
Other | 3 | 9% |
Unknown | 8 | 23% |
Readers by discipline | Count | As % |
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Chemistry | 7 | 20% |
Biochemistry, Genetics and Molecular Biology | 7 | 20% |
Agricultural and Biological Sciences | 5 | 14% |
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Medicine and Dentistry | 2 | 6% |
Other | 5 | 14% |
Unknown | 7 | 20% |