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Mendeley readers
| Chapter title |
Application of Fluorescence Lifetime Imaging (FLIM) to Measure Intracellular Environments in a Single Cell
|
|---|---|
| Chapter number | 8 |
| Book title |
Multi-Parametric Live Cell Microscopy of 3D Tissue Models
|
| Published in |
Advances in experimental medicine and biology, January 2017
|
| DOI | 10.1007/978-3-319-67358-5_8 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-967357-8, 978-3-31-967358-5
|
| Authors |
Takakazu Nakabayashi, Kamlesh Awasthi, Nobuhiro Ohta |
| Abstract |
Fluorescence lifetime imaging (FLIM) has now been used in many bioscience fields, which comes from the quantification of fluorescence lifetime. The procedure for obtaining lifetime images is very similar to that used in fluorescence microscopy. However, obtaining reliable lifetime images requires an understanding of the theory of fluorescence lifetime, principle of FLIM systems, and evaluation procedure of intracellular environments. In this chapter, the materials, methods, and notes on FLIM measurements have been described, in conjunction with a brief explanation of the background of FLIM. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Professor > Associate Professor | 3 | 27% |
| Student > Doctoral Student | 1 | 9% |
| Student > Ph. D. Student | 1 | 9% |
| Professor | 1 | 9% |
| Researcher | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 3 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 45% |
| Agricultural and Biological Sciences | 1 | 9% |
| Physics and Astronomy | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Unknown | 3 | 27% |