| Chapter title |
Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues
|
|---|---|
| Chapter number | 5 |
| Book title |
Multi-Parametric Live Cell Microscopy of 3D Tissue Models
|
| Published in |
Advances in experimental medicine and biology, January 2017
|
| DOI | 10.1007/978-3-319-67358-5_5 |
| Pubmed ID | |
| Book ISBNs |
978-3-31-967357-8, 978-3-31-967358-5
|
| Authors |
Anastasia Shpichka, Anastasia Koroleva, Daria Kuznetsova, Ruslan I. Dmitriev, Peter Timashev, Shpichka, Anastasia, Koroleva, Anastasia, Kuznetsova, Daria, Dmitriev, Ruslan I., Timashev, Peter |
| Abstract |
Polymeric, ceramic and hybrid material-based three-dimensional (3D) scaffold or matrix structures are important for successful tissue engineering. While the number of approaches utilizing the use of cell-based scaffold and matrix structures is constantly growing, it is essential to provide a framework of their typical preparation and evaluation for tissue engineering. This chapter describes the fabrication of 3D scaffolds using two-photon polymerization, decellularization and cell encapsulation methods and easy-to-use protocols allowing assessing the cell morphology, cytotoxicity and viability in these scaffolds. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 16% |
| Student > Bachelor | 5 | 14% |
| Student > Ph. D. Student | 4 | 11% |
| Student > Doctoral Student | 2 | 5% |
| Other | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 17 | 46% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 7 | 19% |
| Engineering | 3 | 8% |
| Biochemistry, Genetics and Molecular Biology | 2 | 5% |
| Agricultural and Biological Sciences | 1 | 3% |
| Chemical Engineering | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 21 | 57% |