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Mendeley readers
| Chapter title |
Fluorescent Labeling of Proteins
|
|---|---|
| Chapter number | 6 |
| Book title |
Single Molecule Analysis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7271-5_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7270-8, 978-1-4939-7271-5
|
| Authors |
Mauro Modesti |
| Abstract |
Many single-molecule experimental techniques exploit fluorescence as a tool to investigate conformational dynamics, molecular interactions, or track the movement of proteins in order to gain insight into their biological functions. A prerequisite to these experimental approaches is to graft one or more fluorophores on the protein of interest with the desired photophysical properties. Here, we describe procedures for efficient methods used to covalently attach fluorophores to proteins. Alternative direct and indirect labeling strategies are also described. |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 17% |
| Student > Bachelor | 4 | 13% |
| Student > Postgraduate | 3 | 10% |
| Researcher | 3 | 10% |
| Student > Doctoral Student | 2 | 7% |
| Other | 5 | 17% |
| Unknown | 8 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 12 | 40% |
| Physics and Astronomy | 4 | 13% |
| Agricultural and Biological Sciences | 2 | 7% |
| Chemistry | 2 | 7% |
| Economics, Econometrics and Finance | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 8 | 27% |