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Mendeley readers
| Chapter title |
Repurposing a Benchtop Centrifuge for High-Throughput Single-Molecule Force Spectroscopy
|
|---|---|
| Chapter number | 19 |
| Book title |
Single Molecule Analysis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7271-5_19 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7270-8, 978-1-4939-7271-5
|
| Authors |
Darren Yang, Wesley P. Wong |
| Abstract |
We present high-throughput single-molecule manipulation using a benchtop centrifuge, overcoming limitations common in other single-molecule approaches such as high cost, low throughput, technical difficulty, and strict infrastructure requirements. An inexpensive and compact Centrifuge Force Microscope (CFM) adapted to a commercial centrifuge enables use by nonspecialists, and integration with DNA nanoswitches facilitates both reliable measurements and repeated molecular interrogation. Here, we provide detailed protocols for constructing the CFM, creating DNA nanoswitch samples, and carrying out single-molecule force measurements. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Researcher | 1 | 17% |
| Student > Postgraduate | 1 | 17% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Unspecified | 1 | 17% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Engineering | 1 | 17% |
| Unknown | 3 | 50% |