Chapter title |
Measurement of Intracellular Ca2+ Concentration in Single Cells Using Ratiometric Calcium Dyes
|
---|---|
Chapter number | 1 |
Book title |
Calcium-Binding Proteins and RAGE
|
Published in |
Methods in molecular biology, January 2013
|
DOI | 10.1007/978-1-62703-230-8_1 |
Pubmed ID | |
Book ISBNs |
978-1-62703-229-2, 978-1-62703-230-8
|
Authors |
Yousang Gwack, Sonal Srikanth |
Abstract |
Measurement of intracellular Ca(2+) concentration ([Ca(2+)](i)) is useful to study the upstream and downstream events of Ca(2+) signaling. Ca(2+)-binding proteins including EF-hand-containing proteins are important downstream effector molecules after an increase of [Ca(2+)](i). Conversely, these proteins can also act as key modulators for regulation of [Ca(2+)](i) by sensing the Ca(2+) levels in the intracellular organelles and cytoplasm. Here we describe a single-cell Ca(2+) imaging technique that was used to measure the intracellular Ca(2+) levels to examine the function of Ca(2+)-binding proteins, STIM1 and Calcium release-activated Calcium channel regulator 2A (CRACR2A), using ratiometric Ca(2+) dye Fura-2 in adherent and non-adherent cells. |
Mendeley readers
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Demographic breakdown
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