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Calcium-Binding Proteins and RAGE

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Cover of 'Calcium-Binding Proteins and RAGE'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Measurement of Intracellular Ca2+ Concentration in Single Cells Using Ratiometric Calcium Dyes
  3. Altmetric Badge
    Chapter 2 Divers Models of Divalent Cation Interaction to Calcium-Binding Proteins: Techniques and Anthology
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    Chapter 3 Probing Ca(2+)-Binding Capability of Viral Proteins with the EF-Hand Motif by Grafting Approach.
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    Chapter 4 New Aspects of Calmodulin–Calmodulin Binding Domains Recognition
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    Chapter 5 Purification and Characterization of the Human Cysteine-Rich S100A3 Protein and Its Pseudo Citrullinated Forms Expressed in Insect Cells
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    Chapter 6 X-ray Structural Analysis of S100 Proteins
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    Chapter 7 Purification and Stable Isotope Labeling of the Calcium- and Integrin-Binding Protein 1 for Structural and Functional NMR Studies
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    Chapter 8 Analysis of Calcium-Induced Conformational Changes in Calcium-Binding Allergens and Quantitative Determination of Their IgE Binding Properties
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    Chapter 9 Longistatin, an EF-Hand Ca2+-Binding Protein from Vector Tick: Identification, Purification, and Characterization
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    Chapter 10 Super-Resolution Microscopy of the Neuronal Calcium-Binding Proteins Calneuron-1 and Caldendrin
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    Chapter 11 NMR Studies of the Interaction of Calmodulin with IQ Motif Peptides.
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    Chapter 12 Biochemical and Immunological Detection of Physical Interactions Between Penta-EF-Hand Protein ALG-2 and Its Binding Partners
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    Chapter 13 Measuring Binding of S100 Proteins to RAGE by Surface Plasmon Resonance
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    Chapter 14 Phage Display Selection of Peptides that Target Calcium-Binding Proteins
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    Chapter 15 RAGE-Mediated Cell Signaling
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    Chapter 16 RAGE Splicing Variants in Mammals
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    Chapter 17 Screening for Novel Calcium-Binding Proteins that Regulate Cardiac Hypertrophy: CIB1 as an Example
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    Chapter 18 In Vivo Screening of S100B Inhibitors for Melanoma Therapy.
  20. Altmetric Badge
    Chapter 19 Arrayed primer extension microarray for the analysis of genes associated with congenital stationary night blindness.
  21. Altmetric Badge
    Chapter 20 Nesfatin-1: Its Role in the Diagnosis and Treatment of Obesity and Some Psychiatric Disorders
  22. Altmetric Badge
    Chapter 21 Human Cell Line Authentication: The Critical First Step in Any Project Using Human Cell Lines
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    Chapter 22 Detection of Ca2+-Binding S100 Proteins in Human Saliva by HPLC-ESI-MS
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    Chapter 23 Clinical Use of the Calcium-Binding S100B Protein
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    Chapter 24 Sensible Use of High-Sensitivity Troponin Assays
  26. Altmetric Badge
    Chapter 25 S100A1 Gene Therapy in Small and Large Animals
Attention for Chapter 1: Measurement of Intracellular Ca2+ Concentration in Single Cells Using Ratiometric Calcium Dyes
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Chapter title
Measurement of Intracellular Ca2+ Concentration in Single Cells Using Ratiometric Calcium Dyes
Chapter number 1
Book title
Calcium-Binding Proteins and RAGE
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-62703-230-8_1
Pubmed ID
Book ISBNs
978-1-62703-229-2, 978-1-62703-230-8
Authors

Yousang Gwack, Sonal Srikanth

Abstract

Measurement of intracellular Ca(2+) concentration ([Ca(2+)](i)) is useful to study the upstream and downstream events of Ca(2+) signaling. Ca(2+)-binding proteins including EF-hand-containing proteins are important downstream effector molecules after an increase of [Ca(2+)](i). Conversely, these proteins can also act as key modulators for regulation of [Ca(2+)](i) by sensing the Ca(2+) levels in the intracellular organelles and cytoplasm. Here we describe a single-cell Ca(2+) imaging technique that was used to measure the intracellular Ca(2+) levels to examine the function of Ca(2+)-binding proteins, STIM1 and Calcium release-activated Calcium channel regulator 2A (CRACR2A), using ratiometric Ca(2+) dye Fura-2 in adherent and non-adherent cells.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 6%
Unknown 15 94%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 25%
Researcher 3 19%
Professor > Associate Professor 2 13%
Student > Master 2 13%
Student > Doctoral Student 1 6%
Other 1 6%
Unknown 3 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 25%
Agricultural and Biological Sciences 2 13%
Engineering 2 13%
Medicine and Dentistry 1 6%
Philosophy 1 6%
Other 2 13%
Unknown 4 25%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2013.
All research outputs
#20,180,477
of 22,694,633 outputs
Outputs from Methods in molecular biology
#9,829
of 13,046 outputs
Outputs of similar age
#248,695
of 280,671 outputs
Outputs of similar age from Methods in molecular biology
#299
of 340 outputs
Altmetric has tracked 22,694,633 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,046 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,671 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 340 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.