Chapter title |
Probing Ca(2+)-Binding Capability of Viral Proteins with the EF-Hand Motif by Grafting Approach.
|
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Chapter number | 3 |
Book title |
Calcium-Binding Proteins and RAGE
|
Published in |
Methods in molecular biology, January 2013
|
DOI | 10.1007/978-1-62703-230-8_3 |
Pubmed ID | |
Book ISBNs |
978-1-62703-229-2, 978-1-62703-230-8
|
Authors |
Zhou Y, Xue S, Chen Y, Yang JJ, Yubin Zhou, Shenghui Xue, Yanyi Chen, Jenny J. Yang, Zhou, Yubin, Xue, Shenghui, Chen, Yanyi, Yang, Jenny J. |
Abstract |
Ca(2+) is implicated in almost every step of the life cycle of viruses, including virus entry into host cells, virus replication, virion assembly, maturation, and release. However, due to the lack of prediction algorithms and rigorous validation methods, only limited cases of viral Ca(2+)-binding sites are reported. Here, we introduce a method to predict continuous EF-hand or EF-hand-like motifs in the viral genomes based on their primary sequences. We then introduce a grafting approach, and the use of luminescence resonance energy transfer and Ca(2+) competition assay for experimental verification of predicted Ca(2+)-binding sites. This protocol will be valuable for the prediction and identification of unknown Ca(2+)-binding sites in virus. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 2 | 40% |
Student > Ph. D. Student | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 40% |
Medicine and Dentistry | 2 | 40% |
Neuroscience | 1 | 20% |