| Chapter title |
A Historical Overview of RNAi in Plants.
|
|---|---|
| Chapter number | 1 |
| Book title |
Antiviral Resistance in Plants
|
| Published in |
Methods in molecular biology, June 2012
|
| DOI | 10.1007/978-1-61779-882-5_1 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-881-8, 978-1-61779-882-5
|
| Authors |
Lindbo JA, John A. Lindbo, Lindbo, John A. |
| Abstract |
RNA interference, or RNAi, is arguably one of the most significant discoveries in biology in the last several decades. First recognized in plants (where it was called post-transcriptional gene silencing, PTGS) RNAi is a gene down-regulation mechanism since demonstrated to exist in all eukaryotes. In RNAi, small RNAs (of about 21-24 nucleotides) function to guide specific effector proteins (members of the Argonaute protein family) to a target nucleotide sequence by complementary base pairing. The effector protein complex then down-regulates the expression of the targeted RNA or DNA. Small RNA-directed gene regulation systems were independently discovered (and named) in plants, fungi, worms, flies, and mammalian cells. Collectively, PTGS, RNA silencing, and co-suppression (in plants); quelling (in fungi and algae); and RNAi (in Caenorhabditis elegans, Drosophila, and mammalian cells) are all examples of small RNA-based gene regulation systems. From the very beginning, plant research has had a major impact on our understanding of RNAi. The purpose of this chapter is to provide an historical perspective and overview on the discovery, characterization, and applications of RNAi in plants. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 67% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | 2% |
| Unknown | 52 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 21% |
| Researcher | 9 | 17% |
| Student > Bachelor | 3 | 6% |
| Professor > Associate Professor | 3 | 6% |
| Professor | 3 | 6% |
| Other | 5 | 9% |
| Unknown | 19 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 26 | 49% |
| Biochemistry, Genetics and Molecular Biology | 8 | 15% |
| Medicine and Dentistry | 1 | 2% |
| Unknown | 18 | 34% |