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Mendeley readers
| Chapter title |
Characterization and Validation of Circular RNA and Their Host Gene mRNA Expression Using PCR
|
|---|---|
| Chapter number | 5 |
| Book title |
Circular RNAs
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7562-4_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7561-7, 978-1-4939-7562-4
|
| Authors |
Andreas W. Heumüller, Jes-Niels Boeckel |
| Abstract |
Polymerase chain reaction enables the detection and characterization of circular RNA expression. The use of divergent primer pairs flanking the back-splice site, being the unique sequence element of a circular RNA, enables the detection of circular RNA expression. Here we describe the basic techniques to detect different circular transcripts of a gene or one circular RNA specifically by PCR and highlight the advantages and drawbacks of both. |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 44% |
| Student > Master | 3 | 17% |
| Professor | 1 | 6% |
| Other | 1 | 6% |
| Researcher | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 4 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 39% |
| Agricultural and Biological Sciences | 2 | 11% |
| Neuroscience | 2 | 11% |
| Medicine and Dentistry | 1 | 6% |
| Environmental Science | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 5 | 28% |