Title |
Chloroplast genome sequencing analysis of Heterosigma akashiwo CCMP452 (West Atlantic) and NIES293 (West Pacific) strains
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Published in |
BMC Genomics, May 2008
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DOI | 10.1186/1471-2164-9-211 |
Pubmed ID | |
Authors |
Rose Ann Cattolico, Michael A Jacobs, Yang Zhou, Jean Chang, Melinda Duplessis, Terry Lybrand, John McKay, Han Chuan Ong, Elizabeth Sims, Gabrielle Rocap |
Abstract |
Heterokont algae form a monophyletic group within the stramenopile branch of the tree of life. These organisms display wide morphological diversity, ranging from minute unicells to massive, bladed forms. Surprisingly, chloroplast genome sequences are available only for diatoms, representing two (Coscinodiscophyceae and Bacillariophyceae) of approximately 18 classes of algae that comprise this taxonomic cluster. A universal challenge to chloroplast genome sequencing studies is the retrieval of highly purified DNA in quantities sufficient for analytical processing. To circumvent this problem, we have developed a simplified method for sequencing chloroplast genomes, using fosmids selected from a total cellular DNA library. The technique has been used to sequence chloroplast DNA of two Heterosigma akashiwo strains. This raphidophyte has served as a model system for studies of stramenopile chloroplast biogenesis and evolution. |
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Geographical breakdown
Country | Count | As % |
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United States | 2 | 3% |
Canada | 2 | 3% |
Portugal | 1 | 1% |
Italy | 1 | 1% |
Germany | 1 | 1% |
Pakistan | 1 | 1% |
Australia | 1 | 1% |
Unknown | 64 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 20 | 27% |
Student > Ph. D. Student | 15 | 21% |
Student > Doctoral Student | 10 | 14% |
Student > Master | 9 | 12% |
Student > Bachelor | 6 | 8% |
Other | 6 | 8% |
Unknown | 7 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 38 | 52% |
Biochemistry, Genetics and Molecular Biology | 13 | 18% |
Environmental Science | 6 | 8% |
Engineering | 2 | 3% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 1% |
Other | 4 | 5% |
Unknown | 9 | 12% |