Chapter title |
Analysis of Metabolites from the Tricarboxylic Acid Cycle for Yeast and Bacteria Samples Using Gas Chromatography Mass Spectrometry
|
---|---|
Chapter number | 20 |
Book title |
Clinical Metabolomics
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7592-1_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7591-4, 978-1-4939-7592-1
|
Authors |
Reza Maleki Seifar, Angela ten Pierick, Patricia T. N. van Dam, Seifar, Reza Maleki, Pierick, Angela, Dam, Patricia T. N. |
Abstract |
We here explain step by step the implementation of gas chromatography coupled with tandem mass spectrometry for the quantitative analysis of intracellular metabolites from the tricarboxylic acid (TCA) cycle such as citrate, isocitrate, alpha-ketoglutarate, succinate, malate, and fumarate. Isotope dilution is used to correct for potential metabolite losses during sample processing, matrix effects, incomplete derivatization, and liner contamination. All measurements are performed in selected reaction monitoring (SRM) mode. Standards and samples are first diluted with a fixed volume of a mixture of fully 13 C-labeled internal standards and then derivatized to give trimethylsilyl-methoxylamine derivatives prior GC-MS/MS analysis. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 12 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 17% |
Student > Bachelor | 2 | 17% |
Lecturer | 1 | 8% |
Professor | 1 | 8% |
Researcher | 1 | 8% |
Other | 0 | 0% |
Unknown | 5 | 42% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 3 | 25% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Immunology and Microbiology | 1 | 8% |
Design | 1 | 8% |
Unknown | 6 | 50% |