| Chapter title |
GC-MS Analysis of Medium- and Long-Chain Fatty Acids in Blood Samples
|
|---|---|
| Chapter number | 18 |
| Book title |
Clinical Metabolomics
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7592-1_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7591-4, 978-1-4939-7592-1
|
| Authors |
Lisa R. Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, Martin Giera |
| Abstract |
Our body contains a wide variety of fatty acids that differ in chain length, the degree of unsaturation, and location of the double bonds. As the various fatty acids play distinct roles in health and disease, methods that can specifically determine the fatty acid profile are needed for fundamental and clinical studies. Here we describe a method for the separation and quantification of fatty acids ranging from 8 to 24 carbon chain lengths in blood samples using gas chromatography-mass spectrometry following derivatization using pentafluorobenzyl bromide. This method quantitatively monitors fatty acid composition in a manner that satisfies the requirements for comprehensiveness, sensitivity, and accuracy. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 24% |
| Student > Master | 3 | 14% |
| Student > Postgraduate | 2 | 10% |
| Student > Bachelor | 2 | 10% |
| Researcher | 2 | 10% |
| Other | 2 | 10% |
| Unknown | 5 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 19% |
| Chemistry | 4 | 19% |
| Immunology and Microbiology | 3 | 14% |
| Agricultural and Biological Sciences | 2 | 10% |
| Neuroscience | 2 | 10% |
| Other | 2 | 10% |
| Unknown | 4 | 19% |