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Noncanonical Amino Acids

Overview of attention for book
Cover of 'Noncanonical Amino Acids'

Table of Contents

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    Book Overview
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    Chapter 1 Leveraging Formylglycine-Generating Enzyme for Production of Site-Specifically Modified Bioconjugates
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    Chapter 2 Artificial Division of Codon Boxes for Expansion of the Amino Acid Repertoire of Ribosomal Polypeptide Synthesis
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    Chapter 3 Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains
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    Chapter 4 Tub-Tag Labeling; Chemoenzymatic Incorporation of Unnatural Amino Acids
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    Chapter 5 Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids
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    Chapter 6 Genetic Code Expansion in Enteric Bacterial Pathogens
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    Chapter 7 Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins
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    Chapter 8 Residue-Specific Incorporation of Noncanonical Amino Acids for Protein Engineering
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    Chapter 9 Using Amber and Ochre Nonsense Codons to Code Two Different Noncanonical Amino Acids in One Protein Gene
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    Chapter 10 Genetic Incorporation of Unnatural Amino Acids into Proteins of Interest in Streptomyces venezuelae ATCC 15439
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    Chapter 11 Expression and Purification of Site-Specifically Lysine-Acetylated and Natively-Folded Proteins for Biophysical Investigations
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    Chapter 12 Site-Specific Incorporation of Sulfotyrosine Using an Expanded Genetic Code
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    Chapter 13 Site-Specific Protein Labeling with Tetrazine Amino Acids
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    Chapter 14 Mapping of Protein Interfaces in Live Cells Using Genetically Encoded Crosslinkers
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    Chapter 15 Generation of Stable Amber Suppression Cell Lines
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    Chapter 16 Trapping Chromatin Interacting Proteins with Genetically Encoded, UV-Activatable Crosslinkers In Vivo
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    Chapter 17 Genetically Encoding Unnatural Amino Acids in Neurons In Vitro and in the Embryonic Mouse Brain for Optical Control of Neuronal Proteins
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    Chapter 18 Genetic Code Expansion- and Click Chemistry-Based Site-Specific Protein Labeling for Intracellular DNA-PAINT Imaging
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    Chapter 19 MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells
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    Chapter 20 Production and Chemoselective Modification of Adeno-Associated Virus Site-Specifically Incorporating an Unnatural Amino Acid Residue into Its Capsid
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    Chapter 21 Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis
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    Chapter 22 Fluorogenic Tetrazine-Siliconrhodamine Probe for the Labeling of Noncanonical Amino Acid Tagged Proteins
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    Chapter 23 Site-Specific Protein Labeling Utilizing Lipoic Acid Ligase (LplA) and Bioorthogonal Inverse Electron Demand Diels-Alder Reaction
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    Chapter 24 Genetic Encoding of Unnatural Amino Acids in C. elegans
Attention for Chapter 3: Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains
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Chapter title
Cell-Free Protein Synthesis for Multiple Site-Specific Incorporation of Noncanonical Amino Acids Using Cell Extracts from RF-1 Deletion E. coli Strains
Chapter number 3
Book title
Noncanonical Amino Acids
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7574-7_3
Pubmed ID
29404990
Book ISBNs
978-1-4939-7573-0, 978-1-4939-7574-7
Authors

Eiko Seki, Tatsuo Yanagisawa, Shigeyuki Yokoyama

Abstract

Cell-free protein synthesis (CFPS) is an effective method for the site-specific incorporations of noncanonical amino acids (ncAAs) into proteins. The nature of in vitro synthesis enables the use of experimental conditions that are toxic or reduce cellular uptake during in vivo site-specific incorporations of ncAAs. Using the Escherichia coli cell extract (S30) from the highly reproductive RF-1 deletion strains, B-60.∆A::Z and B-95.∆A, with orthogonal tRNA and aminoacyl-tRNA synthetase (aaRS) pairs from Methanosarcina mazei, we have developed CFPS methods for the highly productive and efficient multiple incorporation of ncAAs. In this chapter, we describe our methods for the preparation of the S30 and the orthogonal tRNAPyl and PylRS pair, and two CFPS protocols for ncAA incorporation.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 7 50%
Student > Bachelor 2 14%
Student > Ph. D. Student 2 14%
Unknown 3 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 57%
Agricultural and Biological Sciences 2 14%
Chemistry 1 7%
Unknown 3 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 February 2018.
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#20,465,050
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Outputs from Methods in molecular biology
#9,945
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Outputs of similar age from Methods in molecular biology
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