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Mendeley readers
Chapter title |
MultiBacTAG-Genetic Code Expansion Using the Baculovirus Expression System in Sf21 Cells
|
---|---|
Chapter number | 19 |
Book title |
Noncanonical Amino Acids
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7574-7_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7573-0, 978-1-4939-7574-7
|
Authors |
Christine Koehler, Edward A. Lemke |
Abstract |
The combination of genetic code expansion (GCE) and baculovirus-based protein expression in Spodoptera frugiperda cells is a powerful tool to express multiprotein complexes with site-specifically introduced noncanonical amino acids. This protocol describes the integration of synthetase and tRNA gene indispensable for GCE into the backbone of the Bacmid, the Tn7-mediated transposition of various genes of interest, as well as the final expression of protein using the MultiBacTAG system with different noncanonical amino acids. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 13% |
Unspecified | 1 | 13% |
Other | 1 | 13% |
Student > Doctoral Student | 1 | 13% |
Unknown | 4 | 50% |
Readers by discipline | Count | As % |
---|---|---|
Unspecified | 1 | 13% |
Biochemistry, Genetics and Molecular Biology | 1 | 13% |
Immunology and Microbiology | 1 | 13% |
Chemistry | 1 | 13% |
Unknown | 4 | 50% |