Peptide Self-Assembly
Humana Press
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Mendeley readers
| Chapter title |
Preparation of Stable Amyloid-β Oligomers Without Perturbative Methods
|
|---|---|
| Chapter number | 21 |
| Book title |
Peptide Self-Assembly
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7811-3_21 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7809-0, 978-1-4939-7811-3
|
| Authors |
Samuel A. Kotler, Ayyalusamy Ramamoorthy |
| Abstract |
Soluble amyloid-β (Aβ) oligomers have become a focal point in the study of Alzheimer's disease due to their ability to elicit cytotoxicity. A number of recent studies have concentrated on the structural characterization of soluble Aβ oligomers to gain insight into their mechanism of toxicity. Consequently, providing reproducible protocols for the preparation of such oligomers is of utmost importance. The method presented in this chapter details a protocol for preparing an Aβ oligomer, with a primarily disordered secondary structure, without the need for chemical modification or amino acid substitution. Due to the stability of these disordered Aβ oligomers and the reproducibility with which they form, they are amenable for biophysical and high-resolution structural characterization. |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 29% |
| Student > Ph. D. Student | 3 | 14% |
| Student > Bachelor | 3 | 14% |
| Student > Master | 2 | 10% |
| Professor > Associate Professor | 2 | 10% |
| Other | 0 | 0% |
| Unknown | 5 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 19% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 10% |
| Agricultural and Biological Sciences | 2 | 10% |
| Chemical Engineering | 1 | 5% |
| Immunology and Microbiology | 1 | 5% |
| Other | 5 | 24% |
| Unknown | 6 | 29% |