| Chapter title |
Introduction to heavy chain antibodies and derived nanobodies.
|
|---|---|
| Chapter number | 2 |
| Book title |
Single Domain Antibodies
|
| Published in |
Methods in molecular biology, August 2012
|
| DOI | 10.1007/978-1-61779-968-6_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-967-9, 978-1-61779-968-6
|
| Authors |
Vincke C, Muyldermans S, Cécile Vincke, Serge Muyldermans, Vincke, Cécile, Muyldermans, Serge |
| Abstract |
The immune response of infected or immunized dromedaries contains a diverse repertoire of conventional and heavy chain-only antibodies, both functional in antigen binding. By definition, a heavy chain antibody is devoid of a light chain and in the case of the heavy chain antibodies in camelids the CH1 domain is also missing. Consequently a camelid heavy chain antibody associates with its cognate antigen via a single domain, the variable heavy chain domain of a heavy chain antibody or VHH. An antigen-specific VHH, also known as Nanobody, with excellent biochemical properties can be obtained in various ways. Their recombinant expression provides access to user-friendly tools for a wide variety of applications. |
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Geographical breakdown
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|---|---|---|
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| Germany | 1 | <1% |
| Belgium | 1 | <1% |
| Unknown | 209 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 40 | 19% |
| Student > Master | 39 | 18% |
| Researcher | 31 | 15% |
| Student > Ph. D. Student | 30 | 14% |
| Other | 8 | 4% |
| Other | 17 | 8% |
| Unknown | 47 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 59 | 28% |
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| Immunology and Microbiology | 10 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 8 | 4% |
| Engineering | 5 | 2% |
| Other | 30 | 14% |
| Unknown | 52 | 25% |