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Monoclonal Antibodies

Overview of attention for book
Cover of 'Monoclonal Antibodies'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Antigen Production for Monoclonal Antibody Generation
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    Chapter 2 Method for the Generation of Antibodies Specific for Site and Posttranslational Modifications
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    Chapter 3 Immunization, Hybridoma Generation, and Selection for Monoclonal Antibody Production
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    Chapter 4 Hybridoma Technology for the Generation of Rodent mAbs via Classical Fusion
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    Chapter 5 Generation of Rabbit Monoclonal Antibodies
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    Chapter 6 Screening Hybridomas for Cell Surface Antigens by High-Throughput Homogeneous Assay and Flow Cytometry
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    Chapter 7 Screening and Subcloning of High Producer Transfectomas Using Semisolid Media and Automated Colony Picker
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    Chapter 8 Design and Generation of Synthetic Antibody Libraries for Phage Display
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    Chapter 9 Selection and Screening Using Antibody Phage Display Libraries
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    Chapter 10 Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation.
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    Chapter 11 Human B cell immortalization for monoclonal antibody production.
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    Chapter 12 Using Next-Generation Sequencing for Discovery of High-Frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice
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    Chapter 13 Cloning, Reformatting, and Small-Scale Expression of Monoclonal Antibody Isolated from Mouse, Rat, or Hamster Hybridoma
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    Chapter 14 Cloning of Recombinant Monoclonal Antibodies from Hybridomas in a Single Mammalian Expression Plasmid
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    Chapter 15 Monoclonal Antibody Purification by Ceramic Hydroxyapatite Chromatography
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    Chapter 16 Rapid Purification of Monoclonal Antibodies Using Magnetic Microspheres
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    Chapter 17 Generation of Cell Lines for Monoclonal Antibody Production
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    Chapter 18 Expression and Purification of Recombinant Antibody Formats and Antibody Fusion Proteins
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    Chapter 19 Purification of Antibodies and Antibody Fragments Using CaptureSelect ™ Affinity Resins
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    Chapter 20 Reformatting of scFv Antibodies into the scFv-Fc Format and Their Downstream Purification.
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    Chapter 21 Antibody V and C Domain Sequence, Structure, and Interaction Analysis with Special Reference to IMGT ®
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    Chapter 22 Measuring Antibody Affinities as Well as the Active Concentration of Antigens Present on a Cell Surface
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    Chapter 23 Determination of Antibody Structures
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    Chapter 24 Affinity Maturation of Monoclonal Antibodies by Multi-Site-Directed Mutagenesis
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    Chapter 25 Epitope mapping with membrane-bound synthetic overlapping peptides.
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    Chapter 26 Epitope Mapping by Epitope Excision, Hydrogen/Deuterium Exchange, and Peptide-Panning Techniques Combined with In Silico Analysis
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    Chapter 27 Fine Epitope Mapping Based on Phage Display and Extensive Mutagenesis of the Target Antigen
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    Chapter 28 Epitope Mapping with Random Phage Display Library
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    Chapter 29 Epitope Mapping of Monoclonal and Polyclonal Antibodies Using Bacterial Cell Surface Display
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    Chapter 30 Ion Exchange-High-Performance Liquid Chromatography (IEX-HPLC)
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    Chapter 31 Size Exclusion-High-Performance Liquid Chromatography (SEC-HPLC)
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    Chapter 32 N-Glycosylation Characterization by Liquid Chromatography with Mass Spectrometry
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    Chapter 33 Fc Engineering of Antibodies and Antibody Derivatives by Primary Sequence Alteration and Their Functional Characterization
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    Chapter 34 Labeling and Use of Monoclonal Antibodies in Immunofluorescence: Protocols for Cytoskeletal and Nuclear Antigens
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    Chapter 35 Generation and Use of Antibody Fragments for Structural Studies of Proteins Refractory to Crystallization
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    Chapter 36 Antibody array generation and use.
Attention for Chapter 25: Epitope mapping with membrane-bound synthetic overlapping peptides.
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Chapter title
Epitope mapping with membrane-bound synthetic overlapping peptides.
Chapter number 25
Book title
Monoclonal Antibodies
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-62703-992-5_25
Pubmed ID
Book ISBNs
978-1-62703-991-8, 978-1-62703-992-5
Authors

Midoro-Horiuti T, Goldblum RM, Terumi Midoro-Horiuti, Randall M. Goldblum, Midoro-Horiuti, Terumi, Goldblum, Randall M.

Abstract

Epitope mapping with synthetic overlapping peptides is used for identifying epitopes of monoclonal antibodies (mAbs) and antibodies from patient sera (Midoro-Horiuti et al. Mol Immunol 43:509-518, 2006; Ivanciuc et al. J Agric Food Chem 51:4830-4837, 2003; Midoro-Horiuti et al. Mol Immunol 40:555-562, 2003; Wang et al. J Allergy Clin Immunol 125:695-702, 702.e1-702.e6, 2010). When the mAbs recognize epitopes that are also recognized by patients of interest, they may be useful as surrogates for patient antibodies.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 1 50%
Unknown 1 50%
Readers by discipline Count As %
Environmental Science 1 50%
Unknown 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 February 2014.
All research outputs
#15,298,293
of 22,751,628 outputs
Outputs from Methods in molecular biology
#5,313
of 13,088 outputs
Outputs of similar age
#193,105
of 313,461 outputs
Outputs of similar age from Methods in molecular biology
#209
of 658 outputs
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So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
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We're also able to compare this research output to 658 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.