Chapter title |
The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas
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Chapter number | 19 |
Book title |
Germinal Centers
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Published in |
Methods in molecular biology, June 2017
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DOI | 10.1007/978-1-4939-7095-7_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7094-0, 978-1-4939-7095-7, 978-1-4939-7094-0, 978-1-4939-7095-7
|
Authors |
Le Gallou, Simon, Nojima, Takuya, Kitamura, Daisuke, Weill, Jean-Claude, Reynaud, Claude-Agnès, Simon Le Gallou, Takuya Nojima, Daisuke Kitamura, Jean-Claude Weill, Claude-Agnès Reynaud, Gallou, Simon, Gallou, Simon Le |
Abstract |
Expression of activation-induced cytidine deaminase (AID) is the hallmark of B cells engaged in an immune response in germinal centers. We designed an inducible fate-mapping reporter mouse in which AID-expressing B cells could be timely and irreversibly marked, by knockin at the Aicda locus of a tamoxifen-inducible Cre recombinase. This mouse model allows notably for the long-term follow-up of memory B cells and plasma cells engaged in an immune response. We describe here a protocol to generate hybridomas from small memory subsets that can be easily traced and identified in this mouse line through Cre-activated fluorescent reporters. |
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Demographic breakdown
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Members of the public | 2 | 67% |
Scientists | 1 | 33% |
Mendeley readers
Geographical breakdown
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Demographic breakdown
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Student > Doctoral Student | 4 | 27% |
Student > Ph. D. Student | 4 | 27% |
Professor | 2 | 13% |
Student > Master | 1 | 7% |
Researcher | 1 | 7% |
Other | 1 | 7% |
Unknown | 2 | 13% |
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Biochemistry, Genetics and Molecular Biology | 4 | 27% |
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Mathematics | 1 | 7% |
Unknown | 1 | 7% |