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Mendeley readers
| Chapter title |
Intravital Microscopy of T–B Cell Interactions in Germinal Centers
|
|---|---|
| Chapter number | 6 |
| Book title |
Germinal Centers
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7095-7_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7094-0, 978-1-4939-7095-7
|
| Authors |
Changming Shih, Hai Qi, Shih, Changming, Qi, Hai |
| Abstract |
Dynamic imaging analyses of antigen-specific T-B interactions in germinal centers have advanced our understanding of the molecular mechanisms underlying affinity maturation and provided a wealth of information about how follicular helper T cells function in vivo. Here we describe a routine method to visualize fluorescence protein-expressing, antigen-specific T and B cells in germinal centers. The protocol for incorporating functional reporters or genetic perturbation of the T cells by retroviral transduction is also briefly described, using the FRET-based calcium reporter as an example. |
Mendeley readers
The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 22% |
| Student > Doctoral Student | 2 | 22% |
| Researcher | 2 | 22% |
| Lecturer | 1 | 11% |
| Student > Postgraduate | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 1 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 22% |
| Nursing and Health Professions | 2 | 22% |
| Agricultural and Biological Sciences | 1 | 11% |
| Immunology and Microbiology | 1 | 11% |
| Chemistry | 1 | 11% |
| Other | 2 | 22% |