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RNA-Protein Interaction Protocols

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Cover of 'RNA-Protein Interaction Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation of a Sequence-Specific RNA Binding Protein, Polypyrimidine Tract Binding Protein, Using RNA Affinity Chromatography
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    Chapter 2 An Affinity Oligonucleotide Displacement Strategy to Purify Ribonucleoprotein Complexes Applied to Human Telomerase
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    Chapter 3 RNA Affinity Tags for the Rapid Purification and Investigation of RNAs and RNA–Protein Complexes
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    Chapter 4 Assembly and Glycerol Gradient Isolation of Yeast Spliceosomes Containing Transcribed or Synthetic U6 snRNA
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    Chapter 5 Purification of Ribonucleoproteins Using Peptide-Elutable Antibodies and Other Affinity Techniques
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    Chapter 6 CLIP: Crosslinking and ImmunoPrecipitation of In Vivo RNA Targets of RNA-Binding Proteins
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    Chapter 7 Quantitative Analysis of Protein-RNA Interactions by Gel Mobility Shift
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    Chapter 8 Monitoring Assembly of Ribonucleoprotein Complexes by Isothermal Titration Calorimetry
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    Chapter 9 Characterization of RNA—Protein Interactions by Phosphorothioate Footprinting and Its Applications to the Ribosome
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    Chapter 10 In Vivo Analysis of Ribonucleoprotein Complexes Using Nucleotide Analog Interference Mapping
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    Chapter 11 T7 RNA Polymerase-Mediated Incorporation of 8-N 3 AMP Into RNA for Studying Protein-RNA Interactions
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    Chapter 12 RNA-Protein Interaction Protocols
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    Chapter 13 Proteins Specifically Modified With a Chemical Nuclease as Probes of RNA-Protein Interaction
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    Chapter 14 RNA-Protein Crosslink Mapping Using TEV Protease
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    Chapter 15 Structural Analysis of Protein-RNA Interactions With Mass Spectrometry
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    Chapter 16 Analyzing RNA-Protein Crosslinking Sites in Unlabeled Ribonucleoprotein Complexes by Mass Spectrometry
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    Chapter 17 In Vitro Selection of Random RNA Fragments to Identify Protein-Binding Sites Within Large RNAs
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    Chapter 18 Immunoprecipitation Analysis to Study RNA-Protein Interactions in Xenopus Oocytes
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    Chapter 19 Mapping the Regions of RNase P Catalytic RNA That Are Potentially in Close Contact With Its Protein Cofactor
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    Chapter 20 RNA-Protein Interaction Protocols
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    Chapter 21 Analysis of RNA Structure and RNA-Protein Interactions in Mammalian Cells by Use of Terminal Transferase-Dependent PCR
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    Chapter 22 Duplex Unwinding and RNP Remodeling With RNA Helicases
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    Chapter 23 Preparation of Efficient Splicing Extracts From Whole Cells, Nuclei, and Cytoplasmic Fractions
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    Chapter 24 Designing and Utilization of siRNAs Targeting RNA Binding Proteins
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    Chapter 25 The use of Saccharomyces cerevisiae proteomic libraries to identify RNA-modifying proteins
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RNA-Protein Interaction Protocols
Published by
Humana Press, November 2008
DOI 10.1007/978-1-60327-475-3
978-1-58829-419-7, 978-1-60327-475-3

Lin, Ren-Jang

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Mendeley readers

The data shown below were compiled from readership statistics for 131 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 131 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 5%
Researcher 6 5%
Student > Master 5 4%
Student > Bachelor 3 2%
Professor 1 <1%
Other 1 <1%
Unknown 108 82%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 11%
Agricultural and Biological Sciences 5 4%
Neuroscience 2 2%
Business, Management and Accounting 1 <1%
Chemistry 1 <1%
Other 0 0%
Unknown 107 82%