| Chapter title |
Sorghum (Sorghum bicolor)
|
|---|---|
| Chapter number | 14 |
| Book title |
Agrobacterium Protocols
|
| Published in |
Methods in molecular biology, January 2015
|
| DOI | 10.1007/978-1-4939-1695-5_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-1694-8, 978-1-4939-1695-5
|
| Authors |
Xiaomei Guo, Zhengxiang Ge, Shirley J Sato, Tom E Clemente, Shirley J. Sato, Tom E. Clemente, Guo, Xiaomei, Ge, Zhengxiang, Sato, Shirley J., Clemente, Tom E. |
| Abstract |
Agrobacterium-mediated transformation of sorghum (Sorghum bicolor L. Moench) targeting immature embryo explants is a route to introduce transgenic alleles into the crop. The protocol requires maintenance of quality stock plants under greenhouse conditions for a constant supply of immature embryo explants. This is typically carried out by a regular sowing of seeds, minimal use of pesticides, and monitoring of plants to document pollen dispersal and bagging of heads. The time frame from explant inoculation to establishment of a primary transgenic event in the greenhouse typically ranges from 4 to 6 months. Seed set in the primary transformants is comparable to greenhouse-grown stock plants, with the majority of the transgenic alleles being inherited as a single functional locus. |
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Mendeley readers
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