Chapter title |
Assessment of Factor XIII
|
---|---|
Chapter number | 22 |
Book title |
Hemostasis and Thrombosis
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7196-1_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7194-7, 978-1-4939-7196-1
|
Authors |
László Muszbek, Éva Katona, Adrienne Kerényi, Muszbek, László, Katona, Éva, Kerényi, Adrienne |
Abstract |
Blood coagulation factor XIII (FXIII) is essential for maintaining hemostasis. The absence of FXIII results in severe bleeding diathesis, which without prophylaxis frequently leads to fatal bleeding. As the usual hemostasis screening tests remain normal, the diagnosis of FXIII deficiency needs specific tests. Here, we describe FXIII activity determination by the ammonia release assay, which is the first-line test in the diagnostic algorithm for FXIII deficiency. The method for another activity test, the undeservedly rarely used fibrin cross-linking assay, is also presented. Further tests used for the classification of FXIII deficiencies, measurement of FXIII activity in platelets, ELISAs for the measurement of complex plasma FXIII (FXIII-A2B2) antigen and for FXIII-A2 in plasma and platelets are also included. Detailed description of the methods for the detection and measurement of neutralizing auto- and alloantibodies is also provided. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 20% |
Researcher | 2 | 20% |
Student > Ph. D. Student | 2 | 20% |
Student > Doctoral Student | 1 | 10% |
Student > Master | 1 | 10% |
Other | 1 | 10% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 10% |
Unknown | 1 | 10% |