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Brain Development

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Cover of 'Brain Development'

Table of Contents

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    Book Overview
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    Chapter 1 Immunostaining of the developing embryonic and larval Drosophila brain.
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    Chapter 2 Non-fluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila.
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    Chapter 3 Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling.
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    Chapter 4 Flybow to dissect circuit assembly in the Drosophila brain.
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    Chapter 5 Immunofluorescent labeling of neural stem cells in the Drosophila optic lobe.
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    Chapter 6 Using MARCM to Study Drosophila Brain Development.
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    Chapter 7 Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria
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    Chapter 8 Methods in Brain Development of Molluscs
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    Chapter 9 In Situ Hybridization and Immunostaining of Xenopus Brain
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    Chapter 10 Microinjection Manipulations in the Elucidation of Xenopus Brain Development
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    Chapter 11 Morpholino Studies in Xenopus Brain Development
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    Chapter 12 Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification
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    Chapter 13 Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish
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    Chapter 14 Anatomical dissection of zebrafish brain development.
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    Chapter 15 Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain
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    Chapter 16 Transplantation of Neural Tissue: Quail-Chick Chimeras
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    Chapter 17 RNAi-Based Gene Silencing in Chicken Brain Development.
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    Chapter 18 Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development
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    Chapter 19 In Utero Electroporation to Study Mouse Brain Development
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    Chapter 20 The Cre/Lox System to Assess the Development of the Mouse Brain
Attention for Chapter 20: The Cre/Lox System to Assess the Development of the Mouse Brain
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Chapter title
The Cre/Lox System to Assess the Development of the Mouse Brain
Chapter number 20
Book title
Brain Development
Published in
Methods in molecular biology, January 2014
DOI 10.1007/978-1-62703-655-9_20
Pubmed ID
Book ISBNs
978-1-62703-654-2, 978-1-62703-655-9
Authors

Claudius F. Kratochwil, Filippo M. Rijli, Kratochwil CF, Rijli FM

Abstract

Cre-mediated recombination has become a powerful tool to confine gene deletions (conditional knockouts) or overexpression of genes (conditional knockin/overexpression). By spatiotemporal restriction of genetic manipulations, major problems of classical knockouts such as embryonic lethality can be circumvented. Furthermore Cre-mediated recombination has broad applicability in the analysis of the cellular behavior of subpopulations and cell types as well as for genetic fate mapping. This chapter will give an overview about applications for the Cre/LoxP system and their execution.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Italy 1 5%
Unknown 21 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 18%
Student > Bachelor 3 14%
Student > Ph. D. Student 3 14%
Student > Doctoral Student 2 9%
Student > Master 1 5%
Other 3 14%
Unknown 6 27%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 36%
Biochemistry, Genetics and Molecular Biology 6 27%
Immunology and Microbiology 1 5%
Neuroscience 1 5%
Unknown 6 27%