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RNA

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Cover of 'RNA'

Table of Contents

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    Book Overview
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    Chapter 1 The transcriptional landscape.
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    Chapter 2 Working with RNA
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    Chapter 3 Synthesis of RNA by in vitro transcription. - PubMed - NCBI
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    Chapter 4 Efficient Poly(A) + RNA Selection Using LNA Oligo(T) Capture
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    Chapter 5 Genome Browsers
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    Chapter 6 Web-Based Tools for Studying RNA Structure and Function
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    Chapter 7 RNA
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    Chapter 8 Rapid amplification of cDNA ends (RACE).
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    Chapter 9 Fractionation of mRNA Based on the Length of the Poly(A) Tail
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    Chapter 10 Analysis of Mutations that Influence Pre-mRNA Splicing
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    Chapter 11 S1 Nuclease Analysis of Alternatively Spliced mRNA
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    Chapter 12 Promises and challenges in developing RNAi as a research tool and therapy.
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    Chapter 13 Inhibition of Gene Function in Mammalian Cells Using Short-Hairpin RNA (shRNA)
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    Chapter 14 Validation of RNAi by Real Time PCR
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    Chapter 15 Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
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    Chapter 16 The Post-transcriptional Operon
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    Chapter 17 RIP-Chip Analysis: R NA-Binding Protein I mmuno p recipitation-Microarray ( Chip ) Profiling
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    Chapter 18 Isolation of RNP Granules
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    Chapter 19 Electrophoretic Mobility Shift Assay for Characterizing RNA–Protein Interaction
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    Chapter 20 RNA
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    Chapter 21 Prediction of Targets for MicroRNAs
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    Chapter 22 RNA
Attention for Chapter 22: RNA
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Chapter title
RNA
Chapter number 22
Book title
RNA
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-59745-248-9_22
Pubmed ID
Book ISBNs
978-1-58829-913-0, 978-1-59745-248-9
Authors

Henrik Nielsen, Nielsen, Henrik

Abstract

With the development of new technologies for simultaneous analysis of many genes, transcripts, or proteins (the "omics" revolution), it has become common to outsource parts of the experimental work. In order to maintain the integrity of the research projects, it is important that the interphase between the researcher and the service is further developed. This involves robust protocols for sample preparation, an informed choice of analytical tool, development of standards for individual technologies, and transparent data analysis. This chapter introduces some of the problems related to analysis of RNA samples in the "omics" context and gives a few hints and key references related to sample preparation for the non-specialist.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
South Africa 1 8%
Unknown 12 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 38%
Student > Doctoral Student 2 15%
Professor 2 15%
Librarian 1 8%
Researcher 1 8%
Other 2 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 46%
Biochemistry, Genetics and Molecular Biology 3 23%
Environmental Science 1 8%
Economics, Econometrics and Finance 1 8%
Medicine and Dentistry 1 8%
Other 0 0%
Unknown 1 8%