Chapter title |
Blood-Based miRNA Preparation for Noninvasive Biomarker Development.
|
---|---|
Chapter number | 22 |
Book title |
Next-Generation MicroRNA Expression Profiling Technology
|
Published in |
Methods in molecular biology, January 2012
|
DOI | 10.1007/978-1-61779-427-8_22 |
Pubmed ID | |
Book ISBNs |
978-1-61779-426-1, 978-1-61779-427-8
|
Authors |
Svenja Debey-Pascher, Jing Chen, Thorsten Voss, Andrea Staratschek-Jox, Debey-Pascher, Svenja, Chen, Jing, Voss, Thorsten, Staratschek-Jox, Andrea |
Abstract |
This chapter describes several methods for the isolation of miRNAs from peripheral whole blood samples or constituent fractions thereof, such as peripheral blood mononuclear cells, plasma, and serum. The methods described here are recently introduced protocols dedicated to the isolation of total RNAs including small RNAs, e.g., miRNeasy Kit and PAXgene Blood miRNA Kit, or alternatively for the enrichment of low-molecular-weight RNA (LMW RNA) fractions including small RNAs, e.g., using the miRNeasy Kit. Furthermore, modifications of classical RNA purification protocols to facilitate the recovery of small RNAs are highlighted. |
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