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Next-Generation MicroRNA Expression Profiling Technology

Overview of attention for book
Cover of 'Next-Generation MicroRNA Expression Profiling Technology'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Controlling miRNA Regulation in Disease
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    Chapter 2 Introduction to miRNA Profiling Technologies and Cross-Platform Comparison.
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    Chapter 3 Stem-Loop RT-qPCR for MicroRNA Expression Profiling
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    Chapter 4 Poly(T) adaptor rt-PCR.
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    Chapter 5 MicroRNA In Situ Hybridization
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    Chapter 6 Agilent MicroRNA Microarray Profiling System
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    Chapter 7 miRNA Expression Profiling Using Illumina Universal BeadChips
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    Chapter 8 MicroRNA Expression Analysis Using the Affymetrix Platform.
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    Chapter 9 Individualized miRNA Assay Panels Using Optically Encoded Beads
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    Chapter 10 Microfluidic Primer Extension Assay
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    Chapter 11 MicroRNA Profiling Using µParaflo Microfluidic Array Technology.
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    Chapter 12 MicroRNA Expression Analysis Using the Illumina MicroRNA-Seq Platform.
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    Chapter 13 Next-Generation Sequencing of miRNAs with Roche 454 GS-FLX Technology: Steps for a Successful Application.
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    Chapter 14 Methods for small RNA preparation for digital gene expression profiling by next-generation sequencing.
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    Chapter 15 Profiling of Short RNAs Using Helicos Single-Molecule Sequencing.
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    Chapter 16 deepBase: Annotation and Discovery of MicroRNAs and Other Noncoding RNAs from Deep-Sequencing Data.
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    Chapter 17 PhenomiR: MicroRNAs in Human Diseases and Biological Processes
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    Chapter 18 miRNA Expression Profiling: From Reference Genes to Global Mean Normalization
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    Chapter 19 miRNA Data Analysis: Next-Gen Sequencing.
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    Chapter 20 Integrated miRNA Expression Analysis and Target Prediction.
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    Chapter 21 miRNAs in Human Cancer.
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    Chapter 22 Blood-Based miRNA Preparation for Noninvasive Biomarker Development.
Attention for Chapter 22: Blood-Based miRNA Preparation for Noninvasive Biomarker Development.
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Chapter title
Blood-Based miRNA Preparation for Noninvasive Biomarker Development.
Chapter number 22
Book title
Next-Generation MicroRNA Expression Profiling Technology
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-61779-427-8_22
Pubmed ID
Book ISBNs
978-1-61779-426-1, 978-1-61779-427-8
Authors

Svenja Debey-Pascher, Jing Chen, Thorsten Voss, Andrea Staratschek-Jox, Debey-Pascher, Svenja, Chen, Jing, Voss, Thorsten, Staratschek-Jox, Andrea

Abstract

This chapter describes several methods for the isolation of miRNAs from peripheral whole blood samples or constituent fractions thereof, such as peripheral blood mononuclear cells, plasma, and serum. The methods described here are recently introduced protocols dedicated to the isolation of total RNAs including small RNAs, e.g., miRNeasy Kit and PAXgene Blood miRNA Kit, or alternatively for the enrichment of low-molecular-weight RNA (LMW RNA) fractions including small RNAs, e.g., using the miRNeasy Kit. Furthermore, modifications of classical RNA purification protocols to facilitate the recovery of small RNAs are highlighted.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Denmark 1 9%
Unknown 10 91%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 55%
Student > Master 2 18%
Student > Ph. D. Student 1 9%
Student > Bachelor 1 9%
Professor 1 9%
Other 1 9%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 55%
Biochemistry, Genetics and Molecular Biology 3 27%
Chemistry 1 9%
Medicine and Dentistry 1 9%

Attention Score in Context

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#13,856,164
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Outputs from Methods in molecular biology
#5,046
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#168,035
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Outputs of similar age from Methods in molecular biology
#289
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