| Chapter title |
Protein-Coated Microcrystals, Combi-Protein-Coated Microcrystals, and Cross-Linked Protein-Coated Microcrystals of Enzymes for Use in Low-Water Media
|
|---|---|
| Chapter number | 10 |
| Book title |
Enzyme Stabilization and Immobilization
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6499-4_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6497-0, 978-1-4939-6499-4
|
| Authors |
Joyeeta Mukherjee, Munishwar N. Gupta, Mukherjee, Joyeeta, Gupta, Munishwar N. |
| Abstract |
Protein-coated microcrystals (PCMC) are a high-activity preparation of enzymes for use in low-water media. The protocols for the preparation of PCMCs of Subtilisin Carlsberg and Candida antarctica lipase B (CAL B) are described. The combi-PCMC concept is useful both for cascade and non-cascade reactions. It can also be beneficial to combine two different specificities of a lipase when the substrate requires it. Combi-PCMC of CALB and Palatase used for the conversion of coffee oil present in spent coffee grounds to biodiesel is described. Cross-linked protein-coated microcrystals (CL-PCMC) in some cases can give better results than PCMC. Protocols for the CLPCMC of Subtilisin Carlsberg and Candida antarctica lipase B (CAL B) are described. A discussion of their applications is also provided. |
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