Chapter title |
Immunophotoaffinity labeling of the binding proteins for 1-methyladenine, an oocyte maturation-inducing hormone of starfish.
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Chapter number | 22 |
Book title |
Developmental Biology of the Sea Urchin and Other Marine Invertebrates
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Published in |
Methods in molecular biology, February 2014
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DOI | 10.1007/978-1-62703-974-1_22 |
Pubmed ID | |
Book ISBNs |
978-1-62703-973-4, 978-1-62703-974-1
|
Authors |
Kida T, Matsuda S, Kuyama A, Toraya T, Tetsuo Kida, Shinjiro Matsuda, Atsushi Kuyama, Tetsuo Toraya, Kida, Tetsuo, Matsuda, Shinjiro, Kuyama, Atsushi, Toraya, Tetsuo |
Abstract |
Starfish oocytes are naturally arrested at the prophase stage of the first meiotic division and resume meiosis in response to 1-methyladenine (1-MeAde), the oocyte maturation-inducing hormone of starfish. Putative receptors for 1-MeAde have not yet been characterized biochemically, although the specific binding of 1-MeAde to the isolated cortices of starfish oocytes was reported so far. Based on the structure-activity relationship of 1-MeAde analogs, we have designed a photoaffinity labeling reagent. The photoaffinity labeling of oocyte membrane fractions, followed by immunoblotting analysis with anti-1-MeAde antibody, results in the detection of an almost single protein band. This 1-MeAde-binding protein might be a possible candidate of the maturation-inducing hormone receptor of starfish. |
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