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RNA-RNA Interactions

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Cover of 'RNA-RNA Interactions'

Table of Contents

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    Book Overview
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    Chapter 1 Identifying RNA Recombination Events and Non-covalent RNA-RNA Interactions with the Molecular Colony Technique.
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    Chapter 2 RNA-RNA Interactions
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    Chapter 3 RNA–RNA SELEX
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    Chapter 4 Identification of Antisense RNA Stem-Loops That Inhibit RNA-Protein Interactions Using a Bacterial Reporter System.
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    Chapter 5 Transactivation of Large Ribozymes
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    Chapter 6 Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies
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    Chapter 7 Single Molecule Studies of RNA-RNA Interactions.
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    Chapter 8 Modification interference analysis of the ribosome.
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    Chapter 9 Assessing Intermolecular RNA:RNA Interactions Within a Ribonucleoprotein Complex Using Heavy Metal Cleavage Mapping.
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    Chapter 10 Electrophoretic Mobility Shift Assays: Analysis of tRNA Binding to the T Box Riboswitch Antiterminator RNA
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    Chapter 11 Fluorescence Anisotropy: Analysis of tRNA Binding to the T Box Riboswitch Antiterminator RNA
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    Chapter 12 Electrophoretic Mobility Shift Assay of RNA-RNA Complexes.
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    Chapter 13 Structural Studies of a Double-Stranded RNA from Trypanosome RNA Editing by Small-Angle X-Ray Scattering
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    Chapter 14 Fusion RNAs in Crystallographic Studies of Double-Stranded RNA from Trypanosome RNA Editing
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    Chapter 15 ERRATUM: Transactivation of Large Ribozymes
Attention for Chapter 12: Electrophoretic Mobility Shift Assay of RNA-RNA Complexes.
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Chapter title
Electrophoretic Mobility Shift Assay of RNA-RNA Complexes.
Chapter number 12
Book title
RNA-RNA Interactions
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-1896-6_12
Pubmed ID
Book ISBNs
978-1-4939-1895-9, 978-1-4939-1896-6
Authors

Geunu Bak, Kook Han, Kwang-Sun Kim, Younghoon Lee, Kwang-sun Kim

Abstract

A simple, rapid, and sensitive electrophoretic mobility shift assay (EMSA) can be successfully used to analyze RNA-RNA interactions. The EMSA of RNA-RNA complexes can be further used to evaluate the specificity of interactions using competitor RNAs in combination with their mutated versions or nonspecific RNAs, such as yeast tRNA. RNA is simply prepared by in vitro transcription from PCR product templates. Detailed experimental descriptions for EMSA-based analysis of specific RNA-RNA interactions between Sib RNAs and ibs mRNAs as a representative example are presented.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 29 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 14%
Student > Master 4 14%
Student > Bachelor 3 10%
Researcher 2 7%
Student > Postgraduate 2 7%
Other 3 10%
Unknown 11 38%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 7 24%
Agricultural and Biological Sciences 7 24%
Decision Sciences 1 3%
Medicine and Dentistry 1 3%
Chemistry 1 3%
Other 1 3%
Unknown 11 38%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 October 2014.
All research outputs
#20,241,019
of 22,768,097 outputs
Outputs from Methods in molecular biology
#9,865
of 13,090 outputs
Outputs of similar age
#295,626
of 352,897 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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